基于核磁共振的代谢组学在糖尿病中的应用

作为系统生物学的重要组成之一,代谢组学特别适用于糖尿病的研究.核磁共振(NMR)凭借其简便、无偏向性、信息量大、无损伤性等优点成为代谢组学中最重要的分析手段之一.通过对糖尿病生物样品(尿液、血液、唾液、组织提取液等)的NMR分析,可以获得其中内源性代谢产物(乳酸、肌酐、氨基酸、脂蛋白等)的综合信息,从而反映精尿病的病理代谢和生化反应规律.该方法已应用于糖尿病并发症的诊断、降糖药物的作用考察,以及糖尿病致病机制的研究等方面,具有广阔的发展前景.

Nuclear magnetic resonance-based metabonomics and its application for diabetes

As an important component of systemic biology,metabonomics is particularly well-suited for diabetes.Nuclear magnetic resonance (NMR),with its advantages of simple,comprehensive,informative,and non-destructive,becomes an important analytical technique in metabonomic research.By analyzing NMR spectra of diabetic biological samples (urine,blood,saliva,tissue extract liquid,etc),we can obtain integrated information of endogenous metabolites (lactate,creatinine,amino acids,lipoprotein,etc) and illustrate abnormal metabolic response.NMR-based metabonomics approach has been applied to the diagnosis of diabetic complications,the investigation of drug'S function and pathogenic mechanism,and has a promising future.