定量RT-PCR检测结肠癌患者门静脉血CK20 mRNA的表达

目的 检测结肠癌患者门静脉血中细胞角蛋白20（CK20）mRNA的表达水平,并探讨其临床价值。方法 采用实时荧光定量RT-PCR检测31例病理学确诊的结肠癌患者门静脉血和20例健康对照者外周血CK20mRNA的表达水平。结果 结肠癌患者中CK20mRNA阳性率为25.81%,而对照组阳性率为5%,两者相比差异有统计学意义（P〈0.025）。随着临床分期的具体化,患者门静脉血中CK20mRNA表达率随之提高,但各分期间阳性率相比无显著统计学意义（P〉0.05）。DukesLC期与D期患者中〉10拷贝/ml者高于A期与B期患者。结论 术中检测结肠癌患者门静脉血CK20mRNA表达水平,对判断肝脏微转移有一定的提示意义。

Significance of CK20 mRNA expression in portal venous blood of colon cancer detected by quantitative RT-PCR

Objective To detect the expression of cytokeratin 20(CK20) mRNA in the portal venous blood of colon cancer and to discuss the its clinical value. Methods Realtime fluorescent quantitative RT-PCR was used to detect the CK20 mRNA expression in the portal venous blood of 31 patients with colon carcer and 20 healthy volunteers. Results 25.81% of the patients showed CK20 mRNA positive expression in patients colon cancer, while it was 5% in the control group (P< 0.025). With the progress with Duke's stages, the expression level of CK20 mRNA was increased, but in the positive rate comparisonbetween every stages,there was no statistic significance (P >0.05). In the patients positive rate was over 10 copies/ml, the stages of Duke's C and D were higher than those stages of Duke's A and B. Conclusion The expression levl with CK20 mRNA of portal venous blood is detected for patients with colon cancer during operation.It reveals a definitive significance to judge the micrometastasis of liver.