不同管径的TiO2纳米管对人牙周膜细胞的毒性研究

目的：研究不同管径的阳极氧化TiO2纳米管对人牙厨膜细胞（HPDLCs）的毒性效应。方法：采用阳极氧化法在钛基底表面制备不同管径的TiO2纳米管，扫描电镜观察其表面的微结构，肌动蛋白染色研究细胞在材料表面的粘附生长情况，活／死细胞双染色研究对原代培养的HPDLCs的毒性效应。结果：HPDLCs的肌动蛋白骨架在30nm管径的TiO2纳米管表面比70nm和120nm管径的TiO2纳米管更规律，培养24h后，70nm和120nm管径的TiO2纳米管对HPDLCs的毒性显著大于30nm管径的TiO2纳米管。结论：30nm管径的TiO2纳米管有利于HPDLCs的肌动蛋白组装，同时毒性效应最小，有利于HPDLCs的粘附和增殖。

Cytotoxic Effects of Human Periodontal Ligament Fibroblasts on Different TiO2 Nanotube Layers

Objective: To evaluate the cytotoxic of the human periodontal ligament fibroblasts(HPDLCs) on different TiO2 nanotube layers in vitro.Methods: The TiO2 nanotube layers were grown by anodization.SEM experiment method has been employed to characterize the microstructures of the nanotubes.The actin cytoskeletons of cells were visualized by a confocal laser scanning microscopy.Cytotoxicity of HPDLSc on TiO2 nanotube was evaluated by the Live/Dead Double Stain Kit.Results: Actin cytoskeleton of HPDLCs cultured on the 30 nm diameter TiO2 nanotubes seemed to show more regular arrangement than the 70 nm and 120 nm diameter TiO2 nanotubes.The cytotoxic of the HPDLCs on the 30 nm diameter TiO2 nanotubes was significantly lower than the 70 nm and 120 nm diameter TiO2 nanotubes.Conclution: Actin cytoskeleton of cells cultured on the 30 nm diameter TiO2 nanotubes showed more arrangement,and the 30 nm diameter TiO2 nanotubes is most advantageous for adhesion and proliferation of the HPDLCs.