| PCR-DGGE初步分析双胞胎儿童牙菌斑细菌群落组成 |
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| 引用本文： | 陆剑,相艳,李宇红,杜民权. PCR-DGGE初步分析双胞胎儿童牙菌斑细菌群落组成[J]. 口腔医学研究, 2012, 28(10): 997-999 |
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| 作者姓名： | 陆剑 相艳 李宇红 杜民权 |
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| 作者单位： | 武汉大学口腔医学院口腔生物医学教育部重点实验室 湖北武汉 430079 |
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| 基金项目： | 国家自然科学基金资助项目(编号:81170954) |
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| 摘 要： | 目的:应用PCR-DGGE技术对双胞胎儿童牙菌斑细菌群落组成进行初步分析.方法:牙菌斑样本取自6对双胞胎儿童,年龄3.5～5.5岁,共17份.分别提取细菌总DNA,PCR扩增16S rDNA V3可变区,产物经DGGE指纹图谱分析其组成.结果:DGGE图谱显示,无龋和患龋双胞胎儿童牙菌斑菌属分布存在差异.结论:PCR-DGGE技术可较直观、灵敏地反应牙菌斑菌属的组成情况.
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| 关 键 词： | 牙菌斑 细菌群落 变性梯度凝胶电泳 16S rRNA |
The Preliminary Analysis of Microflora in Dental Plaque of Twin Children by PCR-DGGE |
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| Affiliation: | LU Jiang,XIANG Yan,LI Yu-hong,et al.School of Stomatology,Wuhan University,Key Lab of Oral Medicine,MOST,Wuhan 430079 |
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| Abstract: | Objective: To detect bacterial community in dental plaque of twin children by PCR-DGGE.Methods: A total of 17 samples were collected from 6 twins.PCR-based denaturing gradient gel electrophoresis surveys microbial diversity by displaying PCR-generated 16S rDNA V3 fragment that migrate at different distances,reflecting the differences sequence of the fragment.Result: DGGE fingerprint patterns of dental plaque samples showed variation among them.Conclusion: PCR-DGGE method is a sensitive and effective approach to resolve the microflora structure of dental plaque. |
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| Keywords: | Dental plaque Bacterial flora 16S rRNA Denaturing gradient gel electrophoresis |
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