Wntl蛋白和β-eatenin蛋白在乳腺浸润性导管癌中的表达及意义

【摘要】目的探讨Wntl蛋白和13-catenin蛋白在乳腺浸润性导管癌发生发展中的作用及其与临床病理特征的关系。方法应用免疫组化SP法检测Wntl和B—catenin蛋白在15例乳腺癌癌旁组织、25例乳腺增生组织和80例乳腺浸润性导管癌组织中的表达；采用SPSS17．0统计软件包进行分析，计数资料用X。检验，相关性分析用Spearman检验，以仅=0．05为检验水准。结果乳腺癌组Wntl的阳性表达率和β-catenin的异常表达率分别为62．5％（50／80）、68．8％（55／80），显著高于增生组[28％（7／25）、20％（5／25）]和癌旁组[13．33％（2／15）、13．33％（2／15）]，差异均具有统计学意义（P之0．01）。乳腺浸润性导管癌中Wntl的阳性表达和8一catenin的异常表达均与淋巴结转移有关（X2=5．10，4．87，P〈0．05），B—catenin的异常表达还与乳腺浸润性导管癌的组织学分级有关（）（2=5．61，P〈0．05）。乳腺浸润性导管癌中Wntl和B-catenin蛋白表达呈正相关（r=0．313，P〈0．05）。结论Wntl蛋白高表达与8-catenin蛋白异常表达在乳腺癌变过程中起着重要作用，并与淋巴结转移有密切关系。

Expression and significance of Wntl and 15-catenin proteins in breast invasive ductal carcinoma

[ Abstract] Objective To investigate the expressions of Wntl andβ-catenin proteins in mammary cancer and its relationship with clinical pathological characteristics, and explore its role in the pathogenesis of breast cancer. Methods Immunoshistochemisty （SP method） was used to detect the expressions of Wntl and β -catenin proteins in adjacent tissue of breast cancer （ n = 15）, breast hyperplasia tissues （ n = 25）, and breast in vasive ductal carcinoma （ n = 80）. SPSS 17.0 statistical software was used for analy- sis, such as count data with X2 test and correlation analysis with Spearman test, with significance level of ct =0.05. Results The positive rate of Wntl and β-catenin in breast cancer group [62. 5% （50/80） and 68.8 （55/80） ] was significantly higher than that in hyperplasia group [ 28% （7/25） and 20% （5/25） ] and in borderline group [ 13.33 （2/15） and 13.33 （2/15） ] with a significant difference （ P 〈 0.01 ）. Positive expression of Wntl and abnormal expression of β -catenin in breast invasive ductal carcinoma were related to lymphatic metastasis （X2 = 5.10, 4. 87, P 〈 0. 05 ）. Abnormal expression of β -catenin was also related to the histological grading of breast invasive ductal carcinoma （ X2 = 5.61, P 〈 0. 05 ）. The expres- sions of Wntl and 13 -catenin proteins in invasive ductal carcinoma showed a positive correlation （ r = 0. 313, P 〈 0. 01 ）. Conclusions The high level expression of Wntl protein and the abnormal expression of 13-catenin protein might play an important role in breast carcinogenesis, and was related to lymph node metastasis.