高迁移率族蛋白-1在小鼠溃疡性结肠炎发病中的作用研究

目的探讨高迁移率族蛋白-1（HMGBl）在溃疡性结肠炎（UC）发病机制中的作用。方法BALB／c小鼠32只，随机分成2组，即UC模型组（H=24）和正常对照组（n=8）。以3％葡聚糖硫酸钠（DSS）制备小鼠UC模型，造模第24h、96h、168h，分别处死造模组（各8只）；对照组正常饮水。观察其病理变化；ELISA法检测血清HMGBl水平；Western—blot法检测结肠组织HMGBl蛋白的表达。结果随着造模时间的延长，UC组织学评分上升，造模168h时其结肠黏膜表现与人类UC相类似。UC组血清HMGBl水平在造模24h时比正常组略升高[（4．49±0．53）μg／L比（5．09±0．61）μg/L，P〉0．05]，在96h达高峰[（14．74±0．60）μg／L，P〈0．01]，168h时峰值下降[（9．03±0．78）μg／L，P〈0．01]。UC组小鼠结肠组织HMGBl蛋白表达水平在造模24h时较正常略升高（0．49±0．03比0．56±0．02，P〉0．05），在造模96h明显升高（0．76±0．03，P〈0．05），至168h时仍维持在较高水平（0．77±0．04，P〈0．05）。结论HMGB1在UC小鼠血清中水平升高，结肠组织中也表达明显增强，提示HMGB1作为晚期炎症因子可能参与UC的疾病过程。

Role of high mobility group box chromosomal protein 1 in pathogenesis of ulcerative colitis in mice

Objective To establish ulcerative colitis(UC)model in BALB/c mice and to investigate the role of high mobility group box chromosomal protein 1(HMGBI)in pathogenesis of UC.Methods Thirty-two BALB/c mice were randomly divided into UC group(n=24,which were fed with 3%dextran sulfate 80dium solution)and control group(n=8,which were fed with water).The animals were sacrificed at 24.96 and 1 68 hours,respectively,to collect samples of colon and blood.The sernm level of HMGB1 was measured with ELISA and the expression of HMGB1 in colon was determined by Western blotting analysis.Results Histological scoring increased with the induction of the model,and manifestation of colon mucosa at 168h was similar with that of UC in human.The serum level of HMGB1 was slightly higer at 24 h than that of control(5.09±0.61 μg/L vs 4.49±0.53μg/L,P＞0.05),and reached a peak at 96 h (14.74±0.60 μg/L,P＜0.01),decreased at 168 h(9.03±0.78μg/L,P＜0.01).The expression 0.05).significandy increased at 96h(0.76±0.03,P＜0.05)and at 168 h(0.77±0.04,P＜0.05).Conclusion HMGB1 might be involved in pathologic changes of UC at a later stage.