Study on theCorrelation of Plasma NO, ET-1 and ALT in the Patients with Chronic Hepatitis andCirrhosis

Nitric oxide ( NO) is a small molecular sub-stance with many biological functions.NO is an un-stable molecule with free radical( or group) struc-ture. Its half life in vivo is 1 to 5s and is reduced tothe stable terminal products( NO2 -/NO3 -) quickly.Atpresent,many researchers reported that the levelof NO is related with the degree of liver disease. Wedetermined the levels of NO,endothelin- 1 ( ET- 1 )and ALT in the in- patients with chronic hepatitis Band active cirrhosisand analyze t…

Study on the correlation of plasma NO, ET-1 and ALT in the patients with chronic hepatitis and cirrhosis

Summary The levels of plasma nitric oxide (NO), endothelin-1 (ET-1) and ALT in the patients with chronic hepatitis B and active cirrhosis and the correlation among them were observed and analyzed. NO₃ ⁻ was restored by using cadmium column assay and NO₂ ⁻ measured by heavy nitrogen assay. The primitive NO₃ ⁻ and total restored NO₂ ⁻ (NO₃ ⁻ / NO₂ ⁻) in plasma of the patients with chronic hepatitis and cirrhosis. Plasma ET-1 and ALT levels were determined by using radioimmunological assay and Lai’s assay, respectively. Compared with normal control group, the plasma levels of NO₂ ⁻ /NO₃ ⁻ and ET-1 in the patients with chronic active hepatitis and active cirrhosis were significantly increased (P < 0.05 – 0.01). There was a positive correlation between NO and ALT, and ET-1 and ALT in the patients with chronic active hepatitis and active cirrhosis respectively. It was suggested that elevation of both NO and ET-1 levels were closely related with injury severity of liver function.