HPLC法测定正天丸和正天胶囊中羟基红花黄色素A的含量 |
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引用本文: | 汪建君,李玲玲,陈惠玲,黄澜.HPLC法测定正天丸和正天胶囊中羟基红花黄色素A的含量[J].中国药品标准,2013,14(3):197-200. |
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作者姓名: | 汪建君 李玲玲 陈惠玲 黄澜 |
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作者单位: | 汪建君 (厦门市药品检验所,厦门,361012); 李玲玲 (厦门市药品检验所,厦门,361012); 陈惠玲 (厦门市药品检验所,厦门,361012); 黄澜 (厦门市药品检验所,厦门,361012); |
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摘 要: | 目的:建立HPLC法测定正天丸和正天胶囊中羟基红花黄色素A的含量。方法:以十八烷基硅烷键合硅胶为填充剂,以甲醇-乙腈-0.7%磷酸溶液(26∶2∶72)为流动相,检测波长为403 nm。结果:羟基红花黄色素A在6.038 4~150.96μg·mL-1范围内呈良好的线性关系;平均回收率为100.0%,RSD=0.8%(n=6)。结论:本方法准确、快速、重现性好,可用于测定正天丸、正天胶囊中羟基红花黄色素A的含量。
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关 键 词: | 高效液相色谱法 正天丸 正天胶囊 羟基红花黄色素A |
收稿时间: | 2012/12/21 0:00:00 |
HPLC Determination of Hydroxysafflor Yellow A in Zhengtian Pills and Zhengtian Capsules |
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Authors: | Wang Jianjun Li Lingling Chen Huiling and Huang Lan |
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Institution: | (Xiamen Institute for Drug Control,Xiamen 361012 ) |
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Abstract: | Objective: To establish a rapid determination of hydroxysafflor yellow A in zhengtian pills and zhengtian capsules. Methods: Using otcadecylsilane bonded silica gel as the stationary phase and a mixture of methanol,acetonitrile and 0. 7% phosphate acid solu-tion (26: 2: 72) as the mobile phase, and the detection wavelength was 403nm. Results:The calibration curves were linear in the range of 6. 0384-150. 96μg·mL^-1 for hydroxysafflor yellow A. the average recovery rate was 100. 0% ,RSD =0. 8% (n =6). Conclusion: The method is simple, accurate, rapid and reproducible, and it can be used to control the contents of zhengtian pills and zhengtian capsules. |
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Keywords: | HPLC zhengtian pills zhengtian capsules hydroxysafflor yellow A |
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