外阴鳞癌组织MDM2 mRNA和p53蛋白表达及其意义

目的探讨外阴鳞癌组织中MDM2 mRNA和p53蛋白的表达及在外阴鳞癌发生、发展中的作用.方法采用RT-PCR法检测31例外阴鳞癌组织中MDM2 mRNA表达情况,利用免疫组化S-P法检测p53蛋白的表达,并与31例癌旁正常外阴组织进行对照.结果在外阴鳞癌组织中MDM2 mRNA表达率为22.6%,明显高于对照组,P<0.05.MDM2表达与组织分化程度无关,P>0.05.外阴鳞癌组织中p53蛋白表达阳性率为58.1%,明显高于对照组.突变型p53在低、中、高分化外阴鳞癌组中的表达率分别为87.5%、64.3%及22.2%,其表达率随着分化程度的降低而升高,低分化组中的表达率明显高于高分化组,P<0.05.MDM2基因扩增与p53蛋白表达呈正相关,P<0.05.结论MDM2基因过表达是外阴鳞癌发生、发展过程中较常见的分子事件;p53基因突变在外阴鳞癌发生、发展中起重要作用.检测p53蛋白可能对估计外阴鳞癌的预后有所帮助.

Expression and Significance of MDM2 mRNA and p53 Protein in Squamous Cell Carcinoma of the Vulva

Objective To investigate the significance of expressions of MDM2 mRNA and p53 protein in squamous cell carcinoma of the vulva (VSCC).Methods Thirty one cases of VSCC and vulvar epithelia adjacent to the tumour were detected for the expressions of MDM2 mRNA and p53 protein with RT PCR and immunohistochemistry S P methods respectively.Results The expressions of MDM2 mRNA and p53 protein in VSCC were 22 6%(7/31) and 58 1%(18/31) respectively.The expression of p53 in lower grade group was much higher than that in higher grade group, P <0 05.There was the correlation between the expression of MDM2 mRNA and p53 protein, P <0 05.Conclusion In VSCC,the proliferation of MDM2 gene is a usual event and the detection of p53 protein may be helpful to assess the prognosis of VSCC.