慢性硝酸镧染毒大鼠肝脏中镧沉积的研究

目的　探讨低剂量硝酸镧 [La(NO3) 3]的慢性毒性及镧在大鼠肝脏中的沉积情况。方法　连续用 0 1、0 2、2 0、10 0和 2 0 0mg kg的La(NO3) 3给大鼠灌胃 6个月后 ,应用透射电镜技术 (TEM)、X射线微区分析 (XMA)和电感耦合等离子体质谱法 (ICP MS)研究镧在大鼠肝脏中的沉积。结果　 2 0 0mg kgLa(NO3) 3组肝细胞内电子密度高的致密体较多 ,大鼠肝脏中镧的含量 ( 3 890ng g蛋白 )与对照组 ( 3 0ng g蛋白 )相比 ,差异有非常显著意义 (P <0 0 1)。结论　镧可进入到肝细胞内 ,并在动物肝脏中的沉积

Study on lanthanum deposit in liver of rats chronically exposed to lanthanum nitrate at low dose

Objective To study deposit of lanthanum in rat liver.Methods Young Wistar rats were divided into six groups,which were fed at dose of 0^1,0^2,2^0,10^0,20^0 mg/kg lanthanum nitrate (La(NO-3)-3) respectively for six months.The metabolic accumulation of lanthanum(La)in rat liver were investigated by transmission electron microscope(TEM),X-ray microanalysis (XMA)and inductively coupled plasma-mass spectrometry(ICP-MS).Results The number of bodies containning high electronic dense gravel-like granules and lysosomes with dense bodies in hepatocytes increased gradually.The contents of La at dose of 20^0 mg/kg La(NO-3)-3( 3 890 ng/g protein)were higher than those of controls(30 ng/g protein,P<0.01).Conclusion La(NO-3)-3 can enter cells.The content of La in the liver increases regularly with increase of dose and time of drug delivery.