肠易激综合征内脏感觉过敏动物模型的建立

目的 内脏感觉过敏是肠易激综合征(IBS)的主要发病机制之一，本文利用大鼠建立一种内脏感觉过敏的动物模型。方法8～21天雌雄Wistar大鼠每天接受不同压力的结肠机械刺激，成年后直肠扩张时记录大鼠腹肌收缩反应(AWR)评分，并进行降结肠的组织学检查。结果 ①新生儿时期接受不同压力结肠机械刺激的大鼠成年后直肠扩张时AWR评分明显高于对照组，不同压力机械刺激组间AWR评分无明显差异，雌性大鼠AWR评分明显高于雄性大鼠。②各组大鼠降结肠常规病理切片未见明显异常。结论 Wistar大鼠在新生儿时期接受的结肠刺激可造成无明显病理改变的慢性内脏感觉过敏模型，雌性大鼠更具有易感性。

A animal model of chronic visceral hypersensitivity in irritable bowel syndrome

Objective Chronic visceral hypersensitivity is one of the most important mechanisms of irritable bowel syndrome. A animal model of chronic visceral hypersensitivity with Wistar rats was established . Methods Neonatal male and female Wistar rats received mechanical colonic stimulation between postnatal days 8 and 21 .The abdominal withdrawal reflex were recorded during colon distention when they became adults. At the end of the experiment, the sections from the descending colons were examined. Results Neonatal rats treated with repetitive colon distention showed a significant increase in AWR compared with control rats. No significant change in AWR among different colon distention pressure groups was detected. Compared with male rats,female rats showed a significant increases in AWR. No significant structural damage and mucosa inflammation were obtained in all groups. Conclusions These results demonstrate that the persistent visceral hypersensitivity in the absence of identifiable pathology can be modeled with Wistar rats neonatally treated with persistent colon distention. Furthermore female rats have more susceptibility of visceral hypersensitivity compared with the male.