BPH-1通过分泌前列腺素E2促进前列腺间质细胞芳香化酶的表达

目的：研究前列腺上皮细胞旁分泌对间质细胞芳香化酶表达的影响。方法：用前列腺上皮细胞系（BPH-1, LNCap, DU145, PC3）条件培养液（CM）处理间质细胞，用RT-qPCR和Western blotting检测芳香化酶表达水平。用RT-qPCR和ELISA检测上皮细胞系环氧合酶2（COX-2）的表达及其CM前列腺素E2(PGE2)浓度。用添加COX-2特异性抑制剂NS-398的培养液培养BPH-1，检测其CM和PGE2对间质细胞芳香化酶表达影响。结果：良性前列腺增生上皮细胞系BPH-1 CM能促进间质细胞芳香化酶mRNA和蛋白的表达，而前列腺癌细胞系PC3、DU-145和LNCap对芳香化酶的表达没有影响。BPH-1 COX-2 mRNA表达水平和PGE2分泌水平远高于其它癌细胞系。添加NS-398培养BPH-1的CM，其PGE2浓度明显降低。PGE2可明显诱导间质细胞芳香化酶的表达。结论：BPH-1通过分泌PGE2促进前列腺间质细胞芳香化酶的表达。

BPH-1 induces aromatase expression in prostatic stromal cells through paracrine of PGE2

AIM: To characterize the effect of prostatic epithelial cell paracrine on aromatase expression in prostatic stromal cells. METHODS: Conditioned medium (CM) of prostatic epithelial cell lines (BPH-1, LNCap, DU-145 and PC3) were collected and used to treat prostatic stromal cells. Expression of aromatase was determined by real-time RT-PCR and Western blotting. Expression of cyclooxygenase-2 mRNA in prostatic epithelial cell lines and prostaglandin (PGE2) in CMs were examined by real-time RT-PCR and ELISA, respectively. The CM of BPH-1 cells cultured with NS-398, specific inhibitor of cyclooxygenase-2, were collected, and the effect of NS-398 and PGE2 on aromatase expression was analyzed. RESULTS: CM of human benign prostate hyperplasia epithelial cell line (BPH-1) stimulated expression of aromatase mRNA and protein in stromal cells. But CM of prostate cancer epithelial cell lines (LNCap, DU145, PC3) had no effect on aromatase expression. COX-2 mRNA level in BPH-1 was much higher than that of other cell lines and PGE2 concentration in BPH-1 CM was much higher than that of other CMs. PGE2 concentration of the CM from BPH-1 cultured with NS-398 significantly decreased. CM from BPH-1 cultured with NS-398 failed to stimulate aromatase expression, while PGE2 induced aromatase expression in prostatic stromal cells. CONCLUSION: BPH-1 could induce aromatase expression in prostatic stromal cells through paracrine of PGE2.