肝癌局部树突状细胞和记忆T淋巴细胞与患者预后的关系

的探讨肝细胞癌(以下简称肝癌)局部免疫状况与患者预后的关系。方法选取123份肝细胞癌石蜡标本HE染色,判断癌结节内及癌周淋巴细胞浸润情况。免疫组化方法检测树突状细胞(DC)、记忆T淋巴细胞的数目及分布,双重免疫组化方法检测两者之间的关系,分析局部免疫细胞的类型、数目及分布与患者预后的关系。结果癌结节与癌周淋巴细胞数目、分级与无瘤生存期无显著相关(P=0．054,0．071)。癌结节中一定数目的DC(≥25个／10个高倍视野)与无瘤生存期相关(P=0．000)。癌结节中的记忆T细胞数目与无瘤生存期直接相关(P=0．003),癌结节中DC和记忆T细胞的数目相关(r=0．531,P=0．000)。DC浸润多者无瘤生存率较高(P=0．001)。结论癌结节中：DC浸润多者无瘤生存率较高,提示DC在激活T细胞、诱导免疫反应中的重要性,可作为有效的复发转移预测指标之一。

Relationship between dendritic cells and memory T lymphocytes in tumor site and prognosis of hepatocellular carcinoma

OBJECTIVE: To investigate the relationship between the distribution and quantity of dendritic cells (DCs) and memory T lymphocytes in the tumor site and the prognosis of hepatocellular carcinoma (HCC). METHODS: Hematoxilin and eosin staining was done on 123 specimens of pathologically proven HCC to observe the infiltration of lymphocytes. Immunohistochemistry was used to count the S-100 protein positive DCs and CD45RO antigen positive memory T cells in ten high power fields within a specimen. Double immunohistochemical staining with double enzyme renovated by microwave for S-100 protein and CD45RO antigens was used to observe relationship between the positivity of S-100 protein and positivity of CD45RO antigen. The specimens were divided into 4 groups according to the averaged numbers of DCs and memory T cells: group I with the DCs and T cells both >/= average numbers, group II with the DCs >/= T cells and T cells < average number, group III with the DCs < average number and memory T cells >/= average, and group IV with both DCs and T cells < average numbers. RESULTS: HE staining showed that the grade of intratumoral or peritumoral lymphocytes was not correlated with the tumor-free survival rate (P = 0.054, 0.071, both P > 0.05), the number of DCs in the cancerous node (DC >/= 25/10 high power field) was positively correlated with the tumor-free survival rate (P = 0.005) and the number of T cells in the cancerous node was positively correlated with the tumor-free survival rate (P = 0.003). Double staining method of immunohistochemistry showed that in the cancerous nodes the number of CD-100 positive DCs and the number of CD45RO positive memory T cells was significantly correlated (r = 0.531, P = 0.000). A greater number of DCs in the cancerous nodes showed a longer tumor-free survival rate of the patients (P = 0.000 between group I and group IV, P = 0.009 between group II and group IV, and P = 0.545 between group I and group II). CONCLUSION: The tumor-free survival rate of the patients who had more intratumoral DCs is higher, which indicates that DCs play an important role in activation of T lymphocytes and immune response and can be used as an independent prognostic factor.