Role of Nitric Oxide in Prevention of Cognitive Disorders in Neurodegenerative Brain Injuries in Rats |
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Authors: | E B Manukhina M G Pshennikova A V Goryacheva I P Khomenko S Yu Mashina D A Pokidyshev I Yu Malyshev |
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Institution: | (1) Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia;(2) Moscow State University of Medicine and Dentistry, Moscow, Russia |
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Abstract: | NO synthesis disturbances play an important role in the development of neurodegenerative damage in Alzheimer disease. We previously
showed that adaptation to intermittent hypobaric hypoxia prevents cognitive disturbances in rats with experimental Alzheimer
disease 6]. Here we evaluated the role of NO in cognitive disorders and development of adaptive protection during experimental Alzheimer
disease. Adaptation to hypoxia in rats was performed in a hypobaric pressure chamber at a simulated altitude of 4000 m (4 h
per day for 14 days). Alzheimer disease was simulated by bilateral injections of a toxic fragment of β-amyloid (25–35) into
n. basalis magnocellularis. For evaluation of the role of NO in the development and prevention of memory disorders, the rats received intraperitoneally
either NO-synthase inhibitor Nω-nitro-L-arginin (L-NNA, 20 mg/kg, every other day for 14 days) or NO-donor dinitrosyl iron complex (200 μg/kg daily for 14
days). NO-synthase inhibitor potentiated the damaging effect of β-amyloid, abolished the protective effect of adaptation to
hypoxia, and produced memory disorders in rats similar to those observed during experimental Alzheimer disease. In contrast,
the increase in NO level in the body provided by injections of the NO-donor produced a protective effect against memory disorders
caused by β-amyloid similar to that induced by adaptation to hypoxia. We concluded that reduced NO production in the organism
plays an important role in the development of cognitive disorders produced by injections of β-amyloid, while prevention of
NO deficit by administration of NO-donors or nonpharmacological stimulation of NO synthesis can provide a protective effect
in experimental Alzheimer disease.
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 146, No. 10, pp. 371–375, October, 2008 |
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Keywords: | nitrogen oxide adaptation to hypoxia Alzheimer disease nitrogen oxide donor NO-synthase inhibitor |
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