枸杞多糖对高脂饮食诱导的脂肪肝大鼠模型的影响

目的 观察枸杞多糖(lycium barbarum polysaccharide,LBP)对高脂饮食大鼠非酒精性脂肪肝的影响及可能的机制.方法 32只雄性Wistar大鼠随机分成4组:正常对照组、模型组和LBP高、低剂量组.采用喂高脂饲料复制大鼠非酒精性脂肪肝模型.观察不同处理组大鼠血清ALT、AST、血脂、肝脂质、肝组织超氧化物歧化酶(SOD)活性、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量以及肝脏病理形态学的变化.结果 与非酒精脂肪肝模型组相比,LBP干预组血清ALT下降,以高剂量组明显(P＜0.05),血清总胆固醇(TCh)和LDL-Ch明显下降(P＜0.05),血清HDL-ch呈升高趋势,但无显著性差异(P＞0.05),肝内甘油三酯和TCh含量下降(P＜0.05),肝内脂肪变性和炎症程度均明显减轻(P＜0.05),肝内MDA含量均显著降低(P＜0.05),肝内SOD活性和GSH含量升高,高剂量组明显(P＜0.05).结论 LBP可明显减轻高脂饮食诱导的大鼠非酒精性脂肪肝,其机制可能与其抗氧化作用有关.

Preventive and therapeutic effects of Lycium barbarum polysaccharide on rat nonalcoholic fatty liver induced by high fat feeding

Aim To explore the effects of lycium barbarum polysaccharide(LBP) on rat nonalcoholic fatty liver induced by high fat feeding and its possible mechanism.Methods 32 male Wistar rats were randomly divided into four groups:normal control group,model group,two doses of LBP-treated groups.The rat nonalcoholic fatty liver model was induced fed by high fat diet for 9 weeks.Serum ALT,AST,triglyceride(TG),total cholesterol(TCh),low density lipoprotein-cholesterol(LDL-ch) and high density lipoprotein-cholesterol(HDL-ch),TG,TCh,superoxidedismutase(SOD),GSH and malondialdehyde(MDA)in the liver homogenates of various treated groups were detected.Histopathological changes in the livers of various treated groups were observed.Results Compared with model group,Serum ALT level in rats treated with high dose of LBP was diminished(P<0.05).Serum TCh and LDL-Ch level in the rats treated with LBP was decreased(P<0.05).There was no significant difference between serum HDL-ch level in rats treated with LBP and those in model rats(P>0.05).The content of TG and TCh in the liver tissue homogenates from LBP-treated rats was decreased(P<0.05).The degree of hepatic steatosis and inflammation in rats treated with LBP was significantly reduced(P<0.05).The decrease of the content of MDA,the increase of activities of SOD and the content of GSH in liver tissues were found in LBP-treated group(P<0.05).Conclusion LBP markedly reduced rat nonalcoholic fatty liver induced by high fat feeding.The effect of LBP may be associated with the reduced levels of lipid peroxidation in liver tissues.