松针提取物对肾性高血压大鼠的治疗效果观察

目的]探讨松针提取物对两肾一夹(2k1c)大鼠高血压的影响及其机制。方法]建立2k1c高血压大鼠模型,随机分为模型组、阳性药物组、松针提取物高剂量组、松针提取物中剂量组、松针提取物低剂量组、假手术组,各组分别予0.9%氯化钠(Na Cl)溶液、1kg/L的卡托普利悬浊液、50、30、20 kg/L的松针提取物、0.9%Na Cl溶液,连续灌胃4周,监测血压、检测血清中一氧化氮(NO)和血浆中血管内皮素(ET)变化。结果]与模型组相比,松针提取物各剂量组大鼠收缩压(SBP)和舒张压(DBP)均有下降,尤其SBP效果显著,差异有统计学意义(P0.05);其血浆中ET含量下降,血清中NO含量升高,差异有统计学意义(P0.05)。与阳性药物组相比,血压下降较缓慢,幅度较小,ET含量下降及NO含量升高无明显差异。结论]松针提取物通过调节血管内舒缩因子平衡达到降血压效果。

Effect observation of PNE on treating renal hypertension rats

Objective] To investigate antihypertensive effect and possible mechanism of the Pineneedle Extract(PNE) in 2-kidney-1-clip(2k1c) hypertensive animal model. Methods] Established the 2k1c hypertensive animal models. The rats were divided randomly into six groups: model group, western medicine group(Captopril), PNE high-dose group, PNE medium-dose group, PNE low-dose group and sham-operation group. Model group and sham-operation group both received 0.9% sodium chloride. Western medicine group received 1 kg/L Captopril suspensions. PNE high-dose group, PNE medium-dose group, PNE low-dose group received PNE solution, 5 kg/L, 3 kg/L, 2 kg/L, separately. Each group rats was given corresponding drugs by gavage for 4 weeks. Then monitor their blood pressure, measure serum nitric oxide(NO) and plasma endothelin(ET) content. Results] Compared with model group, the PNE each groups rats of diastolic pressure(DBP) and systolic pressure(SBP) both reduced, especially SBP, which were statistically significant(P<0.05). The level of serum NO fell, while the level of plasma ET raised, which were statistically significant(P<0.05). Contrasted to western medicine group, the PNE each group rats of blood pressure decrease slowly. Both the decline of ET content and the rise of NO content were not statistically significant. Conclusion] PNE can reduce blood pressure by adjusting endothelium-derived relaxing and contracting factor.