沉默EMI2基因抑制肝内胆管癌HuCCT1细胞的增殖和上皮间质转化

目的:探索早期有丝分裂抑制子2(early mitotic inhibitor 2,EMI2)在肝内胆管癌(intrahepatic cholan-giocarcinoma,iCCA)中的表达及生物学功能,并探讨EMI2对iCCA细胞HuCCT1增殖、侵袭及上皮-间质转化(epithelial-mesenchymal ...

Silencing EMI2 gene inhibits proliferation and epithelied-mesenchymal transformation of intrahepatic cholangiocarcinoma HuCCT1 cells

Objective:To explore the expression and biological function of early mitotic inhibitor 2(EMI2)in intrahepatic cholangiocarcinoma(iCCA)tissues.To investigate the effects of EMI2 on the proliferation,invasion and epithelial-mesenchymal transformation of iCCA cells HuCCT1.Methods:Bioinformatics technique was used to detect the expression of EMI2 in bile duct carcinoma.Gene silencing technique was used to silence EMI2,and its effects on proliferation,invasion,migration and EMT of HuCCT1 cells were observed.Results:Bioinformatics showed that EMI2 was highly expressed in cholangiocarcinoma tissues,while Western Blot and RT-PCR were ineffective in detecting EMI2.CCK-8 showed that the OD₄₅₀ value of shEMI2 group was lower than that of shCtrol group(P<0.05),and the number of shEMI2 cell clone formation(26±2)was lower than that of shCtrol group(57±4).Invasive results showed that the number of invasion in shEMI2 group was(39±4.92)and in shCtrol group was(195±14.00).Cell cycle test showed that compared with shCtrol group,shEMI2 group of G₂/M cells decreased and G₁ cells increased(P<0.05).The apoptosis experiment showed that the apoptosis rate of shEMI2 group was increased(P<0.05).Western Blot and immunofluorescence results suggested that silencing EMI2 could promote the up-regulation of E-cadherin protein and down-regulation of N-cadherin and Vitmentin protein.Conclusion:Silencing EMI2 gene inhibites the proliferation,invasion,migration and epithelied-mesenchymal transformation of intrahepatic cholangiocarcinoma.