Glypican-6在胃肠道间质瘤组织中的表达及其通过ERK1/2通路促进细胞的增殖、迁移及侵袭

目的:探究Glypican-6对胃肠道间质瘤的作用及其可能的作用机制.方法:收集胃肠道间质瘤组织及其癌旁组织27例,RT-PCR和Western blot检测组织中Glypican-6的表达.选取胃肠道间质瘤细胞系GIST-T1进行体外研究.RT-PCR实验检测细胞中Glypican-6 mRNA的表达;Western...

The expression of Glypican-6 in gastrointestinal stromal tumor tissues and it promotes the proliferation,migration and invasion of cells through the ERK1/2 pathway

Objective:To explore the effect of Glypican-6 on gastrointestinal stromal tumors and its possible mechanism.Methods:Twenty-seven cases of gastrointestinal stromal tumor tissues and adjacent tissues were collected,and the expression of Glypican-6 in the tissues was detected by RT-PCR and Western blot.The gastrointestinal stromal tumor cell line GIST-T1 was selected for vitro studies.RT-PCR experiment was used to detect cell Glypican-6 mRNA expression.Western blot was used to detect cell Glypican-6,E-cadherin,N-cadherin,p-ERK1/2 and ERK1/2 protein expression.CCK-8 was used to detect cell proliferation.Transwell test was used to detect cell migration and invasion.Results:Compared with adjacent tissues,mRNA and protein expression of Glypican-6 in cancer tissues were significantly increased(P<0.05).Compared with the si-NC group,the expression of Glypican-6 mRNA and protein in si-Glypican-6 cells were significantly reduced(P<0.05).The proliferation capacity of cells at 24 h,48 h and 72 h,number of migrating cells and number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly reduced(P<0.05),and the expression of E-cadherin protein was significantly increased(P<0.05).Compared with the pcDNA3.1 group,the expression of Glypican-6 mRNA and protein in the cells of pcDNA3.1-Glypican-6 group were significantly increased(P<0.05),and the proliferation ability at 24 h,48 h and 72 h,number of migrating cells,number of invasion cells,the expression of N-cadherin protein and p-ERK1/2/ERK1/2 rate were significantly increased(P<0.05),and the expression of E-cadherin protein was significantly decreased(P<0.05).Compared with the pcDNA3.1-Glypican-6 group,the pcDNA3.1-Glypican-6+PD98059 group had significantly lower proliferation ability at 24 h,48 h and 72 h(P<0.05),and the number of migrating cells,the number of invading cells and the protein expression of N-cadherin and p-ERK1/2/ERK1/2 rate were significantly reduced(P<0.05),protein expression of E-cadherin was significantly increased(P<0.05),and protein expression of Glypican-6 was not significantly different(P>0.05).Conclusion:Glypican-6 is highly expressed in gastrointestinal stromal tumors.Glypican-6 promotes the proliferation,migration,invasion and protein expression of N-cadherin of gastrointestinal stromal tumor cells through ERK1/2 pathway,and inhibits the expression of E-cadherin protein.