miR-206、FAM83A在膀胱尿路上皮癌组织中的表达及临床意义

目的:探讨微小RNA-206（microRNA-206，miR-206）、83序列相似成员A（family with sequence similarity 83 member A，FAM83A）在膀胱尿路上皮癌（bladder urothelial carcinoma，BUC）组织中的表达及临床意义。方法:选取2015年02月至2017年02月在本院肿瘤外科进行手术治疗的86例BUC患者为研究对象，选择患者癌组织及癌旁组织分别作为BUC组和对照组。采用实时荧光定量PCR（qRT-PCR）法检测miR-206、FAM83A mRNA相对表达量；免疫组化法检测癌组织及癌旁组织中FAM83A的表达情况；分析miR-206、FAM83A表达水平与BUC患者临床病理特征的关系；Kaplan-Meier法分析miR-206、FAM83A表达水平与BUC患者3年生存期的关系。结果:BUC组中miR-206表达水平明显低于对照组（P＜0.05），FAM83A mRNA表达水平明显高于对照组（P＜0.05）。免疫组化结果显示，BUC组中FAM83A阳性率高于对照组（P＜0.001）。BUC组织中miR-206、FAM83A表达与患者肿瘤分期、淋巴结转移、组织学分级、肿瘤直径密切相关（P＜0.05），而与患者年龄、性别无相关性（P＞0.05）。Kaplan-Meier法分析显示，BUC患者3年累积生存率为52.33%（45/86）。miR-206高表达患者3年累积生存率高于miR-206低表达患者；FAM83A高表达患者3年累积生存率低于FAM83A低表达患者。多因素COX分析显示，miR-206低表达、FAM83A水平偏高是BUC患者不良预后的独立危险因素（HR=2.598、3.342，P＜0.05）。结论:BUC患者癌组织中miR-206低表达、FAM83A高表达，miR-206、FAM83A表达与患者肿瘤分期、淋巴结转移、组织学分级、肿瘤直径及不良预后的发生有关。

Expression and clinical significance of miR-206 and FAM83A in bladder urothelial carcinoma tissues

Objective:To investigate the expression and clinical significance of microRNA-206(miR-206)and family with sequence similarity 83 member A(FAM83A)in bladder urothelial carcinoma(BUC)tissues.Methods:From February 2015 to February 2017,86 patients with BUC who underwent surgical treatment in the surgical oncology department of our hospital were selected as the research objects,and the cancer tissues and adjacent tissues were selected as BUC group and control group respectively.Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect the relative expression of miR-206 and FAM83A mRNA.Immunohistochemistry was used to detect the expression of FAM83A in cancer tissues and adjacent tissues.The relationship between the expression of miR-206 and FAM83A and clinicopathological characteristics was analyzed.The relationship between the expression of miR-206 and FAM83A and the 3-year survival of patients with BUC was analyzed by Kaplan-Meier method.Results:The expression level of miR-206 in BUC group was significantly lower than that in control group(P<0.05),and the expression level of FAM83A mRNA in BUC group was significantly higher than that in control group(P<0.05).Immunohistochemical result showed that the positive rate of FAM83A in BUC group was higher than that in control group(P<0.001).The expression levels of miR-206 and FAM83A in BUC tissues were closely correlated with tumor stage,lymph node metastasis,histological grade and tumor diameter(P<0.05),but not with age and gender(P>0.05).Kaplan-Meier analysis showed that the 3-year cumulative survival rate of patients with BUC was 52.33%(45/86).The 3-year cumulative survival rate of patients with high expression of miR-206 was higher than that of patients with low expression of miR-206.The 3-year cumulative survival rate of patients with high expression of FAM83A was lower than that of patients with low expression of FAM83A.Multivariate COX analysis showed that low expression of miR-206 and high level of FAM83A were independent risk factors for poor prognosis of BUC patients(HR=2.598,3.342,P<0.05).Conclusion:The expression of miR-206 is low and the expression of FAM83A is high in the cancer tissues of BUC patients.miR-206 expression and FAM83A expression are related to tumor stage,lymph node metastasis,histological grade,tumor diameter and adverse prognosis in BUC patients.