HCV核心区多肽对细胞毒T细胞作用的初步观察

目的：探讨HCV感染者体内细胞毒T细胞（CTL）功能缺陷的原因，为深入研究HCV感染的发病机制和指导疫苗研制提供理论和实验依据。方法：采用多肽固相合成法选择性合成HCV核心区多肽，并将皮下注射免疫Balb/c小鼠，用乳酸脱氢酶（LDH）释放实验检测小鼠脾细胞（CTL）活性。结果：经单因素方差分析显示，HCV核心区多肽CPA9（39－74位氨基酸），CPB7（67－76位氨基酸），CPB8（71－80位氨基酸）对小鼠CTL有抑制作用，CPA10（5－23位氨基酸），CPB6（63－72位氨基酸）和CPB2（131－140位氨基酸）对小鼠CTL有增强作用，结论：经初步观察，HCV核心区多肽对CTL有抑制作用及增强作用。

A preliminary study of effects of hepatitis C virus nucleopolypeptides on specific cytotoxic T cells

Objective To explore the mechanism of CTL dysfunction in HCV infected person, to provide a theoretical basis for further understanding the pathogenesis of hepatitis C and the development of HCV vaccines. Methods HCV nucleopolypeptides were selected and synthesized with the method of solid phase synthesis. BALB/c mice were immunized subcutaneously with HCV nucleopolypeptides, and CTL activity of mice was detected by LDH releasing test. Results CTL of mice could be inhibited by HCV nucleopolypeptides residues 39-74, 67-76, 71-80 and enhanced by HCV nucleopolypeptides residues 5 23,63 72,131 140. Conclusion The function of CTL can be suppressed and intensified by different HCV nucleopolypeptides.