阿魏酸钠对乙醇损伤性大鼠肝切片的保护作用及机制

目的:采用精密肝切片技术,研究阿魏酸钠(SF)对乙醇损伤性大鼠肝切片的保护作用及机制。方法:制作大鼠乙醇损伤肝切片模型,观察不同浓度SF对切片培养液中肝损伤标志酶及胞浆苯胺羟化酶(ANH)、乙醇脱氢酶(ADH)活性的影响。结果:乙醇50mmol·L-1作用肝切片4h时,培养液中谷丙转氨酶、谷草转氨酶、乳酸脱氢酶和谷胱甘肽S-转移酶活性明显升高,同时肝切片胞浆ANH活性升高、ADH活性降低。当共处理SF463~1388nmol·L-14h后,培养液中各酶活性显著降低至正常水平,SF浓度增至694nmol·L-1以上时,肝切片胞浆ANH和ADH活性也恢复正常。结论:SF能有效拮抗乙醇所致的肝损伤,其机制与改变乙醇代谢途径有关。

Protection of Sodium Ferulate on Ethanol-induced Hepatotoxicity in Precision Liver-cut Slices of Rats and Its Mechanism

Objective: To study the hepato-protection and mechanism of sodium ferulate (SF), which was extracted from angelica and contains phenolic hydroxyl, on the ethanol-induced hepatotoxicity in rats. Methods: 50 mmol·L~-1 ethanol was given to induce hepatotoxicity in precision liver-cut slices of rats. The activities of glutamic pyruvic transaminase (GPT), glutamic oxaloacetic transaminase (GOT), lactate dehydrogenase (LDH) and glutathione S-transferase (GST) in culture medium, and the activities of aniline hydroxylase (ANH) and alcohol dehydrogenase (ADH) in slice cytosol were measured when different concentrations of SF were co-treated with the ethanol. Results: The activities of GPT, GOT, GST and LDH in the medium increased significantly when the slices were treated with 50 mmol·L~(-1)ethanol for 4 hours. SF with concentrations of 463 ~ 1 388 nmol·L~(-1) could effectively reduce the changes of GTP, GOT, GST and LDH activities in the medium of ethanol-treated slices. Meanwhile, both activities of cytosol ANH and ADH returned to the normal levels when SF concentration increased to be 694 nmol·L~-1. Conclusion: SF can markedly protect liver slices from ethanol-induced hepatotoxicity, which partly results from the modification of ethanol-metablizing pathway in liver.