HPLC法同时测定一清颗粒中黄芩苷等8种有效成分的含量

目的建立RP-HPLC法同时测定一清颗粒中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、黄芩苷、汉黄芩素和盐酸小檗碱8种有效成分的含量。方法采用Kromasil C18色谱柱(250 mm×4.6 mm5,μm),以乙腈-体积分数0.1%磷酸水(三乙胺调pH值至3.0)为流动相,梯度洗脱;检测波长为254 nm,流速1.0 mL.min-1,柱温30℃。结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚、黄芩苷、汉黄芩素和盐酸小檗碱质量浓度分别在1.800～36.20、14.25～285.1、4.500～90.01、.780～35.60、4.300～85.0、48.20～963、1.700～34.00、28.60～572.5 mg.L-1内与峰面积呈良好的线性关系(r≥0.999 1),平均回收率(n=9)分别为96.0%、98.0%、96.6%、97.0%9、7.7%9、7.0%9、7.2%9、8.0%。结论该方法快速简便、灵敏、重现性好,可为一清颗粒的质量控制提供依据。

Simultaneous determination of aloe-emodin, rhein, emodin, physcion, chrysophanol, baicalin, wogonin, berberine hydrochloride in Yiqing granules by HPLC

Objective To establish an HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, physcion, chrysophanol, baicalin, wogonin,berberine hydrochloride in Yiqing granules(traditional chinese medicine). Method Isolation of sample was achieved on a Kromasil C₁₈ column (250 mm × 4.6 mm, 5 μm). A mixture of acetonitrile and 0.1% phosphoric acid (adjust pH = 3.0 with triethylamine) with gradient elution was used as mobile phase. The analytes was detected at 254 nm, and the column temperature was maintained at 30 ℃. Results The linearity ranges of aloe-emodin, rhein, emodin, physcion, chrysophanol, baicalin, wogonin, berberine hydrochloride were 1.800-36.20 mg·L^-1, 14.25-285.1 mg·L^-1, 4.500-90.0 mg·L^-1, 1.780-35.60 mg·L^-1, 4.300-85.0 mg·L^-1, 48.20-963 mg·L^-1, 1.700-34.00 mg·L^-1 and 28.60-572.5 mg·L^-1, respectively (r ≥ 0.999 1). The average recoveries(n = 9) of them were 96.0 %, 98.0 %, 96.6 %, 97.0 %, 97.7 %, 97.0 %, 97.2 % and 98.0 %, respectively. Conclusion The method is fast, simple, sensitive and can be used for the quality control of Yiqing granules.