HPLC法测定辽细辛不同药用部位马兜铃酸IVA的含量

目的建立辽细辛不同药用部位中马兜铃酸IVA的含量测定方法。方法采用HPLC法。色谱柱为Shiseido C18柱(250 mm×4.6 mm5,μm),流动相为甲醇-水-冰醋酸(体积比55∶44∶1),检测波长为252 nm,流速为1.0 mL.min-1。结果马兜铃酸IVA质量浓度在4.720～47.20 mg.L-1内线性关系良好(r=0.999 6,n=6),辽细辛根、根茎和叶中马兜铃酸IVA的平均回收率分别为99.6%9、9.4%9、8.9%(n=6),RSD均小于2.8%。结论方法简便、快速、准确,可为辽细辛不同部位的质量控制提供参考依据。

Determination of aristolochic acid IVA in the different parts of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag. by HPLC

Objective To establish an HPLC method for determination of aristolochic acid IVA in different parts of Asarum heterotropoides Fr. Schmidt Var. mandshuricum (Maxim.) Kitag. Methods The determination was performed on Shiseido C₁₈ column (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-water-glacial acetic acid (V︰V︰V = 55︰44︰1). The detection wavelength was set at 252 nm and the flow rate was 1.0 mL·min^-1. Results The calibration curve was linear for aristolochic acid IVA in the range of 4.720-47.20 mg·L^-1 with the correlation coefficient of 0.999 6 (n = 6). The average recoveries of the root, rhizome and leaves were 99.6%, 99.4% and 98.9% respectively, and relative standard deviations were less than 2.8%. Conclusions The method is simple, accurate and selectivity, and can be used for the quality control of different parts of Asarum heterotropoides Fr. Schmidt var. mandshuricum (Maxim.) Kitag.