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Rapid genome sequencing of RNA viruses |
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| Authors: | Mizutani Tetsuya Endoh Daiji Okamoto Michiko Shirato Kazuya Shimizu Hiroyuki Arita Minetaro Fukushi Shuetsu Saijo Masayuki Sakai Kouji Lim Chang Kweng Ito Mikako Nerome Reiko Takasaki Tomohiko Ishii Koji Suzuki Tetsuro Kurane Ichiro Morikawa Shigeru Nishimura Hidekazu |
| Affiliation: | *National Institute of Infectious Diseases, Tokyo, Japan;†Rakuno Gakuen University, Ebetsu, Japan;‡Sendai Medical Center, Sendai, Japan |
| Abstract: | We developed a system for rapid determination of viral RNA sequences whereby genomic sequence is obtained from cultured virus isolates without subcloning into plasmid vectors. This method affords new opportunities to address the challenges of unknown or untypeable emerging viruses. |
| Keywords: | Emerging infectious virus SARS-CoV West Nile virus rapid determination system whole genome amplification direct sequencing dispatch |
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