低能量激光与集落刺激因子对人脐带血造血细胞的增殖作用

目的研究低能量激光对人脐带血造血细胞的增殖效应,并探讨其与集落刺激因子（ＣＳＦ）效应的关系。方法用铜蒸气激光照射人脐带血造血细胞,体外培养扩增细胞并计数集落细胞数。结果激光加ＣＳＦ对造血细胞粒细胞巨噬细胞集落形成单位（ＧＭＣＦＵｃ）有协同增殖作用,与单用激光照射组、ＣＳＦ刺激组及对照组相比,差异有非常显著意义（Ｐ＜００１）。将经激光照射与未照射脐血造血细胞在液体培养基中培养２周,前者活细胞数较培养前增加５５～１１０倍,后者增加１１～２７倍,再次软琼脂接种培养,仍有集落形成,生长的集落绝对数较液体培养前显著增加,而且激光照射组比未照射组更明显。结论低能量激光照射脐血细胞是体外扩增造血细胞的一种有效方法。

Proliferative effect of low energy laser and colony stimulating factor on human umbilical cord blood hematopoietic cells

Objective To study the proliferative effect of low energy laser on human cord blood hematopoietic cells in vitro and the relationship between the effect of low energy laser and colony stimulating factor (CSF). Methods Human umbilical cord blood hematopoietic cells were irradiated with copper vapour laser and cultured in vitro. The number of colony and cells was calculated. Results Laser irradiation synergized with CSF in expansion of cord blood hematopoietic cell. The GM CFUc number from the group treated with irradiation plus CSF increased significantly as compared with that from other groups including laser irradiation or CSF treatment alone or control ( P <0 01). Reincubation of cord blood cells in the soft agar medium revealed that secondary colonies were formed and the absolute number of colony was obviously increased as compared with that before liquid culture. The number of colony in irradiation group was more than these of non irradiation group. Conclusion Low energy laser irradiation for cord blood stem cells is effective in vitro expansion of cord blood stem cells.