RP-HPLC法同时测定复方环昔滴眼液中两组分的含量

目的:建立同时测定复方环昔滴眼液中盐酸环丙沙星和阿昔洛韦的含量的RP-HPLC法。方法:采用Kromasil C18柱(4.6 mm×250 mm,5μm),以15∶15∶70比例的甲醇-乙腈-0.05 mol.L-1枸橼酸溶液(三乙胺调pH至3.5)为流动相,流速为0.8 mL.min-1;检测波长为253 nm。结果:盐酸环丙沙星和阿昔洛韦的线性范围分别为15.1~241.6μg.mL-1(r=0.9993)和5.1~81.6μg.mL-1(r=0.9998),平均回收率分别为99.7%和99.8%,RSD分别为0.92%和0.65%。结论:本法快速准确,重现性好,适用于复方环昔滴眼液的质量控制。

Simultaneous Content Determination of Two Constituents in Compound Huanxi Eye-Drops by RP-HPLC

Objective To establish a RP-HPLC method that can simultaneously determine the contents of ciprofloxacin hydrochloride and aciclovir in compound huanxi eye-drops.Methods The RP-HPLC was performed on a Kromasil C18 column（4.6 mm×250 mm,5 μm）,the mobile phase was composed of methanol-acetonitrile-0.05 mol·L-1 citric acid solution（adjusted to pH3.0 with triethylamine）with a ratio of 15∶15∶70,the flow rate was 0.8 mL·min-1,the detection wavelength was 253 nm.Results The linear ranges of ciprofloxacin hydrochloride and aciclovir were 15.1～241.6 μg·mL-1（r=0.9993）and 5.1～81.6 μg·mL-1（r=0.9998）,respectively.The average recovery rates were 99.7% and 99.8% with RSD 0.92% and 0.65%,respectively.Conclusion The method is accurate and reliable for the quality control of compound huanxi eye-drops.