肝康栓抗鸭乙型肝炎病毒的实验研究

目的：用鸭乙肝模型研究肝康栓对鸭乙型肝炎病毒（DHBV）的体内抗病毒作用。方法：用DHBV阳性血清感染1日龄的樱桃谷鸭，制备鸭乙型肝炎模型。随机分成5组：肝康栓大、中、小3个剂量治疗组，生理盐水模型组和阿昔洛韦（ACV）对照组，每组12只，均连续给药4周。分别于给药前，给药14天、28天，停药7天时取血清，用real-timePCR法检测鸭血清中DHBV DNA拷贝数的变化情况。结果：肝康栓大剂量组给药14天、28天及停药7天，中剂量组给药14天、28天，小剂量组给药28天，鸭血清中DHBV DNA拷贝数的对数值均较给药前降低（〉2个对数级），差异有统计学意义（P〈0．01）。结论：肝康栓具有有效抑制鸭体内DHBV DNA复制的作用。

Experimental study of Gankang Suppository on duck hepatitis B virus in ducklings

Objective:To study the antiviral effect of Gankang Suppository on duck hepatitis B virus (DHBV) in vivo.Methods: One-day-old Yingtao Gu ducklings infected with positive serum of DHBV were used as the hepatitis B animal model. Positive ducklings were randomly divided into 5 groups: Gankang Suppository large-dose group,medium-dose group,small-dose group, normal saline model group, and ACV control group. Every group had 12 ducklings, and was administered continuously for 4 weeks. The change of serologic DHBV DNA copies were detected by real-time PCR test before treatment, 14 days and 28 days after treatment, and 7 days after withdrawal.Results: After medicated with Gankang Suppository 14 days, 28 days and 7 days after withdrawal, the logarithmic value(log) of DHBV DNA in large-dose group or medium-dose group or small-dose group ducklings' sera were obviously lower than which were before treatment(>2 log),there were significant differences(P<0.01). Conclusion: Gankang Suppository could inhibit the replication of DHBV DNA in ducklings in vivo.