miR-490-3p在胃癌中的表达及其临床价值

目的 探讨miR-490-3p表达水平与胃癌临床病理特征和预后的关系。方法 通过实时荧光定量PCR(qPCR)方法检测80例胃癌患者的癌组织与癌旁正常组织中miR-490-3p的相对表达量，分析其与临床病理特征的关系，采用Kaplan-Meier曲线、Cox比例风险回归模型进行预后分析。结果 miR-490-3p在癌组织中的相对表达量为(0.652±0.072)，低于癌旁正常组织中的(1.259±0.069)，差异有统计学意义(t=40.528,P<0.05);miR-490-3p表达与浸润深度、淋巴结转移及TMN分期显著相关(P<0.05);miR-490-3p低表达和高表达患者无进展生存期差异有统计学意义(23个月vs 30个月，χ²=8.583,P=0.03);miR-490-3p、淋巴结转移及TNM分期是胃癌预后的独立影响因素(HR=2.396、0.472、0.230，均P<0.05)。结论 mi R-490-3p表达降低可促进胃癌的增殖、分化和迁移，可成为胃癌诊断和预后的潜在分子标志物。

Expression of miR-490-3p in gastric cancer and its clinical value

ObjectiveTo investigate the relationship between the expression of miR-490-3p clinicopathological features and prognosis of gastric cancer. MethodsThe relative expression of miR-490-3p in cancer tissues and adjacent normal tissues of 80 patients with gastric cancer were detected by quantitative real-time PCR (qPCR), and the relationship between it and clinicalpathological characteristics was analyzed. Kaplan-Meier curve and Cox proportional risk regression model were used for prognostic analysis. ResultsThe relative expression of miR-490-3p in cancer tissues was (0.652±0.072), which was lower than (1.259±0.069) of normal adjacent tissues, with a statistically significant difference (t=40.528, P<0.05). The expression of miR-490-3p was significantly correlated with the depth of invasion, lymph node metastasis and TMN staging (P<0.05). There was a significant difference in progression free survival between patients with low expression and high expression (23 months vs 30 months, χ²=8.583, P=0.03). miR-490-3p, lymph node metastasis and TNM stage were independent prognostic factors of gastric cancer (HR=2.396, 0.472, 0.230, all P<0.05). ConclusionThe down-regulation of miR-490-3p may promote the proliferation, differentiation and migration of gastric cancer, and may become a potential molecular marker for the diagnosis and prognosis of gastric cancer.