玻璃体腔注射HGF-MSCs对糖尿病大鼠视网膜HGF表达的影响

目的 观察玻璃体腔注射肝细胞生长因子基因修饰的人脐带间充质干细胞(HGF-MSCs)对糖尿病大鼠视网膜组织肝细胞生长因子(HGF)表达的影响,探讨HGF-MSCs对大鼠Ⅱ型糖尿病性视网膜病变的作用.方法 体外分离、培养人脐带间充质干细胞(hUCMSCs)并鉴定.采用感染复数值(MOI)50的慢病毒载体转染hUCMSCs...

Effect of intravitreal injection of HGF-MSCs on the expression of HGF in retina tissue of diabetic rats

Objective To observe the effect of intravitreal injection of human umbilical cord mesenchymal stem cells(hUCMSCs)modified by the hepatocyte growth factor gene(HGF-MSCs)on the hepatocyte growth factor(HGF)expression in retina tissue of diabetic rats, and to explore the effect of HGF-MSCs on diabetic retinopathy in rats. Methods hUCMSCs were isolated, cultured, and identified. hUCMSCs were transfected with lentiviral vectors with a multiplicity of infection(MOI)of 50. Four-week-old Sprague-Dawley rats were randomly divided into the normal(15 rats)and diabetes(60 rats)groups. The diabetic rat models were established by intraperitoneal injection of streptozotocin(STZ). Three months after the diabetic rat models were confirmed successfully, the diabetes group was further divided into the diabetes control(no injection), PBS(intravitreal injection of PBS), MSCs(intravitreal injection of MSCs), and HGF-MSCs(intravitreal injection of HGF-MSCs)groups. After 2, 4, and 6 weeks of intravitreal injection, the HGF mRNA expression in rat retina was detected through real-time PCR. The expression of HGF in the rat retina was assessed through Western blot. Results The cultured hUCMSCs can be differentiated into osteoblasts and adipocytes. MOI 50 lentivirus successfully transfected hUCMSCs. Two weeks after intravitreal injection, quantitative real-time PCR and Western blot showed that, at the same time point, the expression of HGF and HGF mRNA in the normal group decreased compared to that of the other groups, and the difference was statistically significant(P<0.05). Compared with other groups at the same time point, the expression of HGF and HGF mRNA in HGF-MSCs group increased, and the difference was statistically significant(P<0.05). No significant difference in the expression of HGF and HGF mRNA was observed in the same group at different time points(P>0.05). Conclusion The HGF and HGF mRNA expression increased in diabetic rat retina. Intravitreal injection of hUCMSCs did not affect the HGF and HGF mRNA expression in diabetic rat retina. Intravitreal injection of hUCMSCs modified by HGF increased the HGF and HGF mRNA expression in diabetic rats. HGF-MSCs may interact with HGF to alleviate early diabetic retinopathy.