| 生长激素调节T淋巴细胞的功能 |
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| 引用本文： | 邓洁英,史轶蘩,SHI Yi-Fan.生长激素调节T淋巴细胞的功能[J].中国免疫学杂志,2000,16(9):480-484. |
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| 作者姓名： | 邓洁英 史轶蘩 SHI Yi-Fan |
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| 作者单位： | 1. 中国医学科学院中国协和医科大学北京协和医院，北京 100730
2. Peking Union Medical College Hospital, Chinese Academy of Medical Sciencesand Peking Union Medical College, Beijing 100730 |
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| 基金项目： | 国家自然科学基金资助课题(39770350)，该课题在卫生部内分泌重点实验室完成 |
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| 摘 要： | 目的：探讨生长激素（ＧＨ）对Ｔ淋巴细胞功能的影响。方法：构建ＧＨ ｃＤＮＡ表达质粒ＰｃＤＮＡ－ＧＨ ｃＤＮＡ，然后将其转染入Ｔ淋巴细胞系－Ｊｕｒｋａｔ细胞中，观察过度表达的内源ＧＨ和加入外源基因重组人ＧＨ对Ｔ淋巴细胞功能的影响。结果：过量表达的内源ＧＨ使Ｊｕｒｋａｔ细胞表达ＩＬ－２Ｒ和分泌ＩＬ－２，ＩＦＮ－γ分别增加１．４０倍，１．６０倍和１．９８倍，而在培养液中加入抗ＧＨ血清后，ＧＨ的这种作用消
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| 关 键 词： | 生长激素 T淋巴细胞 细胞因子 免疫调节 |
Growth hormone regulate the function of T lymphocytes |
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| WANG Hui,DENG Jie-Ying,SHI Yi-Fan.Growth hormone regulate the function of T lymphocytes[J].Chinese Journal of Immunology,2000,16(9):480-484. |
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| Authors: | WANG Hui DENG Jie-Ying SHI Yi-Fan |
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| Abstract: | To study the effects of growth hormone on function of T lymphocytes. Methods: Tne expression plasmid PcDNA-GHcDNA was constructed,then the plasmids were transfected into Jurkat cells,on the other hand,Jurkat cells were treated with different doses ofrhGH, to investigate the effect of overexpression GH and rhGH on function of T lymphocytes. Results: Overexpression GH could increase the ex-pression of IL-2R(140％) and secretion of IL-2(160％) and IFN-γ( 198% ) in Jurkat cells after PHA activation,but these data were broughtdown by the treatment of GH antibody. rhGH could also enhancer the function of T lymphocytes after PHA activation. Conclusion: These resultssupport the idea that GH is an autocrine and paracrine factor, and can regulate the function of T lymphocytes. |
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| Keywords: | Growth hormone T lymphocyte Cytokine |
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