螺旋藻激酶对急性血瘀模型大鼠的影响

目的 探讨螺旋藻激酶(SPK)对急性血瘀模型大鼠的血液流变学、血小板聚集率和环磷酸腺苷(cAMP)的影响.方法 40只SD大鼠分为正常对照组、模型组、阿司匹林组和SPK高、低剂量组,灌胃给药7 d,采用肾上腺素冰水浴刺激复制急性血瘀大鼠模型,应用全自动血流变测定仪检测全血黏度、血浆黏度、红细胞压积(Hct)、红细胞聚集指数(EAI)、红细胞变形指数(DI)和红细胞刚性指数(ERI),酶联免疫吸附试验(ELISA)测定血浆cAMP的水平,采用二磷酸腺苷诱导大鼠血小板聚集,用比浊法测定最大聚集率.结果 SPK可以降低急性血瘀大鼠的全血黏度,血浆黏度、Hct、EAI和血小板最大聚集率,升高血浆cAMP水平,与模型组比较差异有统计学意义(P<0.05),对DI和ERI无明显影响.结论 SPK能明显改善急性血瘀大鼠血液流变学的异常变化且具有抗血小板聚集作用.

The effects of Spirulina kinase in rats with acute blood stasis

Objective In this study ,the effect of Spirulina kinase(SPK)on hemorheology ,platelet aggregation and cAMP in acute blood stasis rats model were investigated .Methods Forty SD rats were randomly divided into normal control group ,model group ,aspirin group and the high and low dose of SPK groups .All treatments were performed by gavage once a day for 7 days .Sub-cutaneous injection of adrenalin combined with ice water bath were used to establish the acute blood stasis rat model .The whole blood viscosity ,plasma viscosity ,red blood cell hematocrit (Hct) ,erythrocyte aggregation index (EAI) ,red blood cell deformation index(DI)anderythrocyterigidityindex(ERI)weredetectedbyautomaticbloodflow speedanalyzer .Meanwhile,thelevelofcAMP was detected by ELISA method .Rat platelet aggregation induced by ADP and the maximum aggregation rate was measured by tur-bidimetry .Results Results showed that SPK could significantly decrease the whole blood viscosity ,plasma viscosity ,Hct ,EAI and platelet aggregation rate ,increase the level of cAMP compared with model group(P<0 .05) ,but had no effect on DI and ERI .Con-clusion SPK can markedly improve the abnormal changes of hemorheology in acute blood stasis rats and inhibit the platelet aggregation .