Bulk immunopurification and development of a radioimmunoassay for human and murine F liver protein |
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| Authors: | D B Oliveira |
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| Institution: | 1. Department of Pharmaceutical Sciences, College of Pharmacy, Northeast Ohio Medical University, Rootstown, OH, USA;2. Department of Medicine, Division of Gastroenterology, Hepatology and Nutrition, University of Pittsburgh, Pittsburgh, PA, USA;3. Pittsburgh Liver Research Center, University of Pittsburgh, Pittsburgh, PA, USA;1. Faculty of Mechanical Engineering, University of Tabriz, 29thBahman Blvd., Tabriz, Iran;2. Department of Mechanical and Energy Engineering, Shahid Beheshti University, Tehran, Iran;1. Division of Cancer Research and Training, Department of Internal Medicine, Charles Drew University of Medicine and Science, David Geffen UCLA School of Medicine and UCLA Jonsson Comprehensive Cancer Center, Los Angeles, CA 90095, USA;2. Fulgent Life Inc., Irvine, CA 92620, USA;3. Nuklein Pharma Company, Ltd, Shenzhen 518118, China;4. Department of Breast Cancer, Cancer Center, Guangdong Provincial People’s Hospital & Guangdong Academy of Medical Sciences, Guangzhou 510080, China;5. Key Laboratory of Exercise and Health Sciences of Ministry of Education, Shanghai University of Sport, Shanghai 200438, China;6. FuJian Sports Vocational Education and Technical College, Fuzhou 350001, China;1. Yonsei University, School of Mechanical Engineering, 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea;2. Dongguk University, College of Medicine, 32 Dongguk-ro, Ilsandong-gu, Goyangsi, Gyeonggi-do, 10326, Republic of Korea;3. DABOM Inc., 50 Yonsei-ro, Seodaemun-gu, Seoul, 03722, Republic of Korea |
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| Abstract: | By taking advantage of the cross-reaction for human F liver protein (F antigen) of a monoclonal antibody raised to mouse F antigen, human F antigen has been immunopurified in bulk. The molecular weight is 44 000. Using this material as a standard, affinity-purified polyclonal anti-F antibody on a solid phase and radiolabelled monoclonal anti-F antibody to detect bound protein, a capture immunoradiometric assay capable of detecting down to 1 ng X ml-1 of F antigen has been developed. Using this assay, the average level of F antigen in normal mouse serum (CBA strain) is 16 ng X ml-1 (360 pM) and in human serum 10 ng X ml-1 (250 pM). |
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