ATRA诱导肺癌细胞株A549分化与凋亡

目的:体外研究维甲类化合物的衍生物ATRA对A549肺癌细胞株作用,探讨ATRA对肿瘤防治作用的可能机制。方法:取对数生长期的常规培养的肺癌A549细胞用于试验,试验分1×10-5mol/L,1×10-6mol/L,1×10-7mol/L三组药物浓度和对照及空白对照5组,加药48h进行MTT测定,第6 d对1×10-5mol/L,1×10-6mol/L及对照组细胞进行流式细胞检测,并对1×10-5mol/L,1×10-6mol/L浓度进行电镜观察。结果:不同浓度ATRA对细胞增殖有明显地抑制作用,与阴性对照组比较,有统计学意义(P<0.01)。增殖抑制有良好剂量效应关系,(r=0.968),细胞阻滞于G0/G1期,高浓度组电镜下见凋亡细胞。结论:ATRA可诱导A549细胞分化及凋亡,为临床应用ATRA化学预防肺癌提供理论依据。

All-trans-retinoic acid induced differentiation and apoptosis of human lung epithelial carcinoma cell line A549

Objective:To investigate the potential roles and mechanism of all-trans-retinoic acid(ATRA) in human lung epithelial carcinoma cell line A549 in vitro.Methods: Routine culturated A549 cell of logarithmic period of growth was devided into 5 groups: drug concentration of 1×10~(-5)mol/L,1×10~(-6)mol/L,1×10~(-7)mol/L,control group and blank control group.MTT was detected in all groups in 48 hours after adding ATRA,FCM were detected in 1×10~(-5)mol/L,1×10~(-6)mol/L concentraction groups and control group on the 6 day and observe the cells of 1×10~(-5)mol/L,1×10~(-6)mol/L concentration groups by electronic microscopy at the meantime.Results: ATRA of different concentration had obvious inhibition effect on cell proliferation and had statistic significance compared with negative control group(P<0.01).Proliferation inhibition had dose-dependent manner(r=0.968).The cell were blocked in G0/G1 stage.Apoptotic cells were found only in 10 mol/L ATRA group by electronic microscopy.Conclusion: ATRA could both induce differentiation and apoptosis of A549 cells.This observation may provide evidence for ATRA in chemoprevention of lung cancer.