细胞因子在特发性肺间质纤维化血管生成中的作用

目的 通过研究特发性肺间质纤维化（ＩＰＦ）患者开胸肺活检标本中胰岛素样生长因子（ＩＧＦ）－Ⅰ和血小板衍生长因子（ＰＤＧＦ）的表达,进一步阐明它们在ＩＰＦ过程中的作用。方法 采用免疫组化和原位杂交方法,分别利用ＩＧＦ－Ⅰ和ＰＤＧＦ的特异抗体和特异引物,检测其在ＩＰＦ患者开胸肺活检标本中的要布和表达。结果 在ＩＰＦ患者中,ＩＧＦ－Ⅰ主要分布在肺动脉血管、新生血管的平涌肌细胞和内皮细胞。肺泡巨噬细胞、Ⅱ

The potential role of cytokine expression and its relation to angiogenesis in idiopathic pulmonary fibrosis

OBJECTIVE: The expression of insulin-like growth factor (IGF)-I and platelet-derived growth factor (PDGF) in open lung biopsies from patients with idiopathic pulmonary fibrosis (IPF) was studied to investigate their precise role in the development of fibrosis in IPF. METHODS: The distribution and expression of IGF-I and PDGF were examined in lung sections of IPF patients with antibodies and cDNA probes of IGF-I and PDGF by immunohistochemistry and in situ hybridization. RESULTS: IGF-I was predominantly localized in vascular smooth muscle cells and vascular endothelial cells of newly formed vessels in IPF. Smooth muscle cells of pulmonary arteries which underwent hypertrophy and hyperplasia strongly expressed IGF-I (+ + +). Type II cells, alveolar macrophages, and interstitial cells were also stained with anti-IGF-I, but with lesser intensity (+). PDGF-AA and BB were localized in hyperplastic bronchio-alveolar epithelial cells and interstitial fibroblasts (+ + +). Alveolar macrophages, vascular endothelial cells and smooth muscle cells were also stained positive (+ +). CONCLUSION: IGF-I and PDGF promote angiogenesis and vascular smooth muscle cell hypertrophy and hyperplasia, which contribute to the progression of fibrosis in IPF. IGF-I and PDGF are also involved in reepithelization and fibroproliferation in IPF through interaction with mesenchymal cells.