| 重组人内皮抑素的氨基酸组成分析 |
| |
| 引用本文： | 毕华,饶春明,史新昌,李永红,王军志.重组人内皮抑素的氨基酸组成分析[J].药物分析杂志,2007,27(6):857-859. |
| |
| 作者姓名： | 毕华 饶春明 史新昌 李永红 王军志 |
| |
| 作者单位： | 中国药品生物制品检定所 |
| |
| 摘 要： | 目的:应用 Hitachi L-8800型氨基酸分析仪对重组人内皮抑素(rh-endostatin)进行氨基酸组成分析,以为其结构确认提供依据。方法:采用酸水解,以外标法测定样品的氨基酸含量及其组成。结果:测定了 rh-endostatin 样品中15种氨基酸的组成,与其理论值相符,误差在15%以内。结论:以氨基酸自动分析仪对样品进行氨基酸组成分析,重复性好,误差较小,能够为样品的结构确认提供一定的依据。
|
| 关 键 词： | 重组人内皮抑素(rh-endostatin) 氨基酸分析 |
| 文章编号： | 0254-1793（2007）06-0857-03 |
| 修稿时间： | 2006-08-24 |
Amino acid components analysis of recombinant human endostatin |
| |
| BI Hua, RAO Chun - ming, SHI Xin - chang, LI Yong - hong, WANG Jun - zhi.Amino acid components analysis of recombinant human endostatin[J].Chinese Journal of Pharmaceutical Analysis,2007,27(6):857-859. |
| |
| Authors: | BI Hua RAO Chun - ming SHI Xin - chang LI Yong - hong WANG Jun - zhi |
| |
| Institution: | National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China |
| |
| Abstract: | Objective:To analyse amino acid components of recombinant human endostatin(rh-endostatin)so as to provide basis for the characteristic structure by Hitachi L-8800 amino acid analyzer.Method:Hydrochloric acid hydrolysis,external reference method for determining amino acid content and composition of sample.Result:Deter- mined the 15 kinds of amino acid composition of rh-endostatin samples,the measured value was identical with the theoretical value and experimental error was within 15%.Conclusion:Analyzing sample by amino acid analyzer is reproducible,accurate and can provide foundature for structure identification. |
| |
| Keywords: | recombinant human endostatin(rh-endostatin) amino acid analysis |
| 本文献已被 CNKI 维普 等数据库收录！ |
|
