酶电极法测定乳酸钠注射液中L-乳酸钠的含量

 目的：研究测定乳酸钠注射液中L-乳酸钠含量的方法。方法：以固定化L-乳酸氧化酶与H202电极构成酶电极，研究了乳酸（盐）酶电极分析法的各种分析条件及参数，并与非水滴定法对比测定了乳酸钠注射液中L-乳酸钠及D，L-外消旋乳酸钠的含量。结果：供试乳酸钠注射液中L-乳酸钠含量约占乳酸钠总量的10%。结论：乳酸钠注射液属于不等量立体异构体药物。采用酶电极法测定乳酸钠注射液中的L-乳酸钠含量具有专一性高、成本低、分析速度快等优点。

Enzyme electrode for the determination of sodium lactate in sodium lactate injection

OBJECTIVE: To determine the concentration of sodium L-lactate in sodium lactate injection. METHODS: Lactic oxidase was immobilized at a hydrogen peroxide electrode and the enzyme electrode was used for the amperometric determination of sodium L-lactate in sodium lactate injection. Sodium D, L-lactate in injection was analyzed by that technique according to Chinese Pharmacopoeia. RESULTS: The quantity of sodium L-lactate was about 10% in total amount of sodium D, L-lactate in sodium lactate injection. CONCLUSION: Sodium L-lactate in sodium lactate injection could be determined specially, rapidly and inexpensively.