平阳霉素诱导人食管癌细胞EC8712凋亡的研究

目的：以平阳霉素诱导食管癌细胞凋亡，并探索药物浓度与诱导凋亡之间的关系。材料与方法：应用电镜、ＤＮＡ电泳、ＴＵＮＥＬ、荧光显微镜、激光共聚焦扫描微镜等手段，观察食管癌细胞在不同浓度平阳霉素作用不同时间后的变化插征及诱导凋亡之作用。结果：细胞形态学及ＤＮＡ水平的变化均证实靶细胞发生了凋亡，但较大浓度较长时间的平阳霉素作用可致其坏死；平阳霉素对ＥＣ８７１２凋亡的诱导效率呈浓度、时间依赖性增强。结论：一

Pingyangmycin- induced apoptosis in human esophageal carcinoma cells EC8712

Objectives:Pingyangmycin was used in vitro to induce apoptosis in human esophageal carcinoma (EC) cell line EC8712 and the concentration effect relationship was studied. Methods:Transmission electron microscopy (TEM), electrophoresis of DNA, terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL), fluorescence microscopy and confocal laser scanning microscopy (CLSM) were used to analyse the characteristic cellular changes. Results:Pingyangmycin used in proper concentration may induce apoptosis in EC8712, but higher concentration of the drug can lead necrosis of the cells. Pingyangmycin induced apoptosis of EC8712 cells is in a concentration dependent manner. Conclusions:Apoptosis of EC8712 can be induced by pingyangmycin within proper concentration after a long enough time. Apoptosis induction may be the major mechanism in chemotherapy of esophageal cancer with pingyangmycin. It is suggested that pingyangmycin should be used rationally according to the concentration effect relationship in clinical chemotherapy in order to induce the tumor cells to maximal apoptotic rate without necrosis and toxic or side effects.