一种新的血浆中磺胺二甲嘧啶及其代谢物的测定方法及在N-乙酰化分型中应用

 目的:测定血浆中磺胺二甲嘧啶及其代谢物，研究其在体内乙酰化表型。方法:用乙腈直接沉沈血浆蛋白，处理后样品在流动相为乙腈-水-醋酸(16.5:83.5:0.05)，流速1.0 ml·min^-1,紫外测定波长260 nm条件下进样。结果:磺胺二甲嘧啶和乙酰磺胺二甲嘧啶相关性分别为0. 999 9和0. 999 6。方法的平均回收率为(99. 49±3.62)%和(99.89±0.87)%。 120名健康志愿者服用磺胺二甲嘧啶片后，用本方法测定体内药物浓度，得快乙酰化者与慢乙酰化者之比为101:19。结论:方法简单、快速、灵敏，能较好地N-乙酰化酶表型。

A new method for determination of sulfamethazine and N-acetylsulfamethazine in plasma and application in N-acetyltransferase phenotyping

OBJECTIVES: To determine concentrations of sulfamethazine(SM₂)and N-acetylsulfamethazine(AC-SM_{2-1，and the ultraviolet detector was set at 260nm RESULTS: The coefficients of correlation for sulfamethazine and N-acetylsulfamethazine were 0. 999 9 and 0. 999 6 respectively.The average recovery was 99.49% for SM2 and 99. 89% for Ac-SM2 with relative standard deviations of 3. 62 % and 0. 87 % respectively. This method was used to determine metabolite ratio of SM2/AC-SM2 for phenotype of N-acetyltransferase in 120 volunteers after taking SM2 tablets. Nineteen slow acetylators were found among 120 volunteers.CONCLUSION: The method is simple, rapid and sensitive and can be used to determine phenotype of N-aceyltransferase in volunteers.}