补阳还五汤对腓总神经损伤大鼠腓总神经中神经生长因子mRNA表达的影响

目的：探讨补阳还五汤对腓总神经损伤大鼠的腓总神经中神经生长因子（ NGF ）-mRNA 表达的影响。方法选取雄性无特定病原体级雄性SD大鼠48只分模型组、假手术组、弥可保组及补阳还五汤高、中、低剂量组各8只。除假手术组大鼠外，均建立腓总神经夹伤模型，术后假手术组与模型组分别用生理盐水灌胃，弥可保组及各剂量补阳还五汤组分别给予相应药物灌胃，其中补阳还五汤高、中、低剂量组所含生药分别为25．92 mg／g、12．96 mg／g、6．48 mg／g，21 d后检测大鼠腓总神经NGF-mRNA表达情况。结果假手术组、弥可保组与补阳还五汤低、中、高剂量组NGF-mRNA相对表达量明显增高，分别是模型组的4．79倍、2．16倍、1．31倍、1．83倍、3．41倍；补阳还五汤中剂量及高剂量组、弥可保组NGF mRNA 相对表达量明显高于模型组（P＜0．05），补阳还五汤高剂量组NGF mRNA相对表达量明显高于弥可保组（P＜0．05）。结论补阳还五汤可明显增强腓总神经损伤大鼠腓总神经中NGF-mRNA的表达，这可能是补阳还五汤可促进腓总神经损伤后的修复及再生、肢体功能恢复的机制之一。

Effect of Buyanghuanwu decoction on nerve growth factor mRNA expression of common peroneal nerve in rats with common peroneal nerve injury repairing

Objective To explore the effect of Buyanghuanwu decoction on nerve growth factor ( NGF) mRNA expression of common peroneal nerve in rats with common peroneal nerve injury .Methods A total of 48 male specific pathogen free SD rats were divided into model group,sham operation group,methycobal group,high-dose Buyanghuanwu decoction group ,medium-dose Buyanghuanwu decoction group and low-dose Buyanghuanwu decoction group ,with 8 rats in each group .Common peroneal nerve crush models were established in all the rats except the rats in the sham operation group .After operation ,sham operation group and model group were treated with normal saline by gavage,while methycobal group and all Buyanghuanwu decoction groups were given corresponding transgastric medication .And the pure drugs in the high-,medium-and low-dose Buyanghuanwu decoction groups were 25.92 mg/g,12.96 mg/g and 6.48 mg/g,respectively. NGF mRNA expression was determined in the common peroneal nerve of rats 21 days later .Results Relative expression levels of NGF mRNA in the sham group , methycobal group , low-, medium-and high-dose Buyanghuanwu decoction groups increased significantly , and were 4.79 times,2.16 times,1.31 times,1.83 times and 3.41 times the relative expression levels in the model group respectively .Relative expression levels of NGF mRNA in the medium-and high-dose Buyanghuanwu decoction groups and methycobal group were significantly higher than that in the model group (P<0.05),and relative expression level of NGF mRNA in the high-dose Buyanghuanwu decoction group was significantly higher than that in the methycobal group (P <0.05).Conclusion Buyanghuanwu decoction can significantly elevate NGF mRNA expression of common peroneal nerve in rats with common peroneal nerve injury .It may be one of the mechanisms that Buyanghuanwu decoction can promote the repairing and regeneration of injured common peroneal nerve ,and recovery of limb function .