异种神经移植后再生神经纤维的HRP逆行运输

成年青紫蓝兔一侧胫神经于Ｇｕｏ窝上部切除５ｍｍ，移植入８ｍｍ长，经反复冰冻，融解５次的成年狗胫神经，将兔胫神经的近，远断端与植入的狗神经缝合。术后２、３、４、５、６周，将ＨＲＰ注入距远端吻合部１５ｍｍ处的远段胫神经，动物存活４天。于神经移植术后４、５、６周，在兔的Ｌ７，Ｓ１．２脊髓前角观察到ＨＲＰ记细胞。

RETROGRADE TRANSPORTATION OF HRP IN REGENERATING NERVE FIBER AFTER TRANSPLANTATION WITH THE HETEROGRAFT

A segment of 5 mm long was removed from the right tibial nerve proximal to the popliteal fossa of the experimental adult rabbit. Another segment of 8 mm in length excised from the tibial nerve of abult dog and treated by repeated freezing and thawing for five times was transplanted instead. Both the proximal and distal ends of the tibial nerve of the host rabbit were sutured to the graft nerve of the donor dog. HRP was injected into the tibial nerve distal of the graft 2, 3,4,5 and 6 weeks after transplantation. HRP labelled neurons were found in the anterior horn of the spinal cord and the spinal ganglia from L7 to S2 4 , 5 and 6 weeks after transplantation. The number of the labelled neurons increased with prolonged survival time. Regenerating nerve fibers of the host could pass the heterograft nerve and grow into the distal part of the tibial nerve. Axoplasm flow of the regenerating nerve fibers was recovered, which proved that HRP was transported to the neuron bodies. The labelled neurons were also found in the anterior horn of the spinal cord and the spinal ganglia, which indicated that regenerating nerves fibers had motor fibers as well as sensory fibers.