新疆阿魏药材 HPLC 指纹图谱研究 |
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引用本文: | 李超颖,卿德刚,张娟,倪慧,孙宇,张海英,薛洁.新疆阿魏药材 HPLC 指纹图谱研究[J].新疆医学院学报,2014(3):317-319,324. |
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作者姓名: | 李超颖 卿德刚 张娟 倪慧 孙宇 张海英 薛洁 |
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作者单位: | [1] 新疆大学生命科学与技术学院,乌鲁木齐830046 [2] 新疆维吾尔自治区中药民族药研究所,乌鲁木齐830002 [3] 新疆医科大学附属中医医院,乌鲁木齐830002 |
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基金项目: | 基金项目:国家自然科学基金(81060332) |
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摘 要: | 目的:建立新疆阿魏药材 HPLC 指纹图谱的鉴别方法。方法以 Waters XTerra RP18(250 mm×4.6 mm,5μm)色谱柱为分析柱,流动相为乙腈-0.01%磷酸水,采用梯度洗脱,流速为1.0 mL/min,323 nm 波长下检测,对10个不同批次药材的 HPLC 指纹图谱进行比对。结果新疆阿魏药材 HPLC 指纹图谱共有18个共有峰,且具有较高的相似度,平均值为0.980。结论该方法简便,重复性好,特征性强,可用于新疆阿魏药材的鉴别和质量控制。
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关 键 词: | 新疆阿魏 指纹图谱 鉴别 |
Research of Ferula sinkiangensis K .M .Shen by HPLC fingerprint |
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Authors: | LI Chaoying QING Degang ZHANG Juan NI Hui SUN Yu ZHANG Haiying XUE Jie |
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Institution: | LI Chaoying, QING Degang, ZHANG Juan, NI Hui, SUN Yu, ZHANG Haiying, XUE Jie |
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Abstract: | Objective To establish HPLC fingerprint for the identification of Ferula sinkiangensis K .M . Shen .Method The chromatographic separation was conducted on a Waters XTerra RP18 (250 mm × 4.6 mm,5 μm)with a mobile phase of acetonitrile-0.01% phosphoric acid,gradient eluent at the flow rate of 1.0 mL/min.The UV detection was set at 323 nm.Result By comparing 10 batches of different batches of medicines for HPLC fingerprint,there were 18 mutual peaks with a high degree of similarity(with the average of 0.98).Conclusion The method is stable and reliable with a good reproducibility and could serve as a reference standard for the identification and quality control of Ferula sinkiangensis K .M.Shen. |
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Keywords: | HPLC HPLC Fingerprint Identifying |
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