| HPLC法测定四季三黄片中黄芩苷的含量 |
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| 引用本文： | 吴继周,;王燕,;韩海霞. HPLC法测定四季三黄片中黄芩苷的含量[J]. 新疆医科大学学报, 2014, 0(6): 715-716 |
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| 作者姓名： | 吴继周, 王燕, 韩海霞 |
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| 作者单位： | [1] 新疆博尔塔拉蒙古自治州药品检验所,新疆 博乐833400; [2] 新疆维吾尔自治区人民医院药学部,乌鲁木齐830001; [3] 新疆农业大学食品科学与药学学院,乌鲁木齐830052 |
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| 基金项目： | 新疆维吾尔自治区少数民族科技骨干人才特殊培养科研专项基金项目（2004-34） |
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| 摘 要： | 目的：建立四季三黄片中黄芩苷含量的测定方法。方法采用高效液相色谱法（HPLC），色谱柱：Diamonsil C18色谱柱（250 mm ×46． mm ，5μm ）；流动相为02．％磷酸溶液-乙腈（78∶22），梯度洗脱，流速：1 mL/min；检测波长：276 nm ；柱温：22℃；进样量为5μL。结果黄芩苷进样量与峰面积在0.0303∽09．090μg范围内呈良好的线性关系，r ＝09．999；平均回收率为990．8％，RSD为09．9％，3批三黄四季片中黄芩苷的平均含量分别为15．8、23．5、14．9 m g/g。结论该方简便易行，精密度、重现性良好，结果准确，可用于四季三黄片的质量控制。
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| 关 键 词： | 四季三黄片 黄芩苷 含量测定 |
Determination of baicalin in Siji sanhuang tablets by HPLC |
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| Affiliation: | WU Jizhou, WANG Yan, HAN Haixia (1 Xinjiang Bortala Mongol Autonomous Prefecture Institute for Drug Control, Xinjiang Bole, 833400 China; 2Department of Pharmacy, The Peoplels Hospital Xinjiang Uyghur Autonomous Region, Urumqi 830001, China ; 4College of Pharmacy, Xinjiang Agricultural University ,Urumqi 830052 China) |
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| Abstract: | Objective To establish a method for determining the content of baicalin in Siji sanhuang tablets . Methods The Diamonsil C18 （250 mm ＆#215; 4 6. mm ,5 μm） was used ,the 0 .2% phosphate acid-acetonitrile （78∶22） as mobile phase .The detection wavelength was 276 nm ,the flow rate was 1 0. mL/min ,the col-umn temperature was 22℃ and the injection volume was 5 μL .Results The linear range of baicalin was 0.030 3∽0. 909 0 μg （r =0 .999 9） ,the average recovery was 99. 08% and RSD was 0. 99% .Conclusion The method is simple ,accurate and reproducible ,and it can be used for quality control of Siji sanhuang tablets . |
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| Keywords: | HPLC HPLC Siji sanhuang tablets baicalin content determination |
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