| 反相高效液相色谱法测定昆仑雪菊中木犀草素的含量 |
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| 引用本文： | 赵文惠,朱雅琪,李新霞.反相高效液相色谱法测定昆仑雪菊中木犀草素的含量[J].新疆医科大学学报,2014(5):567-569. |
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| 作者姓名： | 赵文惠 朱雅琪 李新霞 |
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| 作者单位： | 新疆医科大学药学院,乌鲁木齐830011 |
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| 基金项目： | 新疆维吾尔自治区自然科学基金（2012211A024） |
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| 摘 要： | 目的：建立昆仑雪菊中木犀草素的含量测定方法。方法采用反相高效液相色谱法测定，色谱柱：Waters XBridge C18（4．6 mm ×250 mm，5μm），流动相 A：乙腈；B：2％冰醋酸；C：水；梯度洗脱程序：0 min，A∶B （15∶85）；10 min，A∶B （20∶80）；15 min，A∶B（35∶65）；30 min，A∶B（15∶85），柱温35℃，检测波长350 nm，流速1．0 mL/min。结果木犀草素在0．1～1．0μg/mL浓度范围内线性关系良好（r ＝0．9999），平均回收率为94．90％，RSD为0．69％。结论该方法简便，结果准确，可以用于昆仑雪菊中木犀草素的含量测定。
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| 关 键 词： | 反相高效液相色谱 昆仑雪菊 木犀草素 |
The determination of the content of luteolin in Kunlun chrysanthemum by HPLC |
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| ZHAO Wenhui,ZHU YaQi,LI Xinxia.The determination of the content of luteolin in Kunlun chrysanthemum by HPLC[J].Journal of Xinjiang Medical University,2014(5):567-569. |
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| Authors: | ZHAO Wenhui ZHU YaQi LI Xinxia |
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| Institution: | (College of Pharmacy, Xinjiang Medical University, Urumqi 830011, China) |
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| Abstract: | Objective To develop an HPLC method for the determination of the content of luteolin in Kun-lun chrysanthemum.Methods The content of luteolin in Kunlun chrysanthemum was determined by HPLC.The exact process was：column：Waters XBridge C18（250 mm×4.6 mm,5μm）;mobile phase A：cetonitrile;B：2%glacial acetic acid（V/V）,gradient elution：0 min,A∶B（15∶85）;10 min,A∶B（20∶80）;15 min,A∶B（35∶65）;30 min,A∶B（15∶85）;the flow rate was 1.0 mL/min,the detection wave-length was at 350 nm,and the column temperature was 35℃.Results Linearity of luteolin was good in the range of 0.1~1μg/mL （r=0.999 9）.The average recovery of luteolin was 94.90% with RSD 0.69%. Conclusion This method is convenient,accurate,and can be used to determine the content of luteolin in Kunlun chrysanthemum. |
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| Keywords: | HPLC Kunlun chrysanthemum luteolin |
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