| 正交优化-微波辅助提取山香圆叶总黄酮的工艺研究 |
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| 引用本文： | 吴珊,李小芳,舒予,刘玲,文怡静,罗佳.正交优化-微波辅助提取山香圆叶总黄酮的工艺研究[J].成都中医药大学学报,2014(2):15-17. |
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| 作者姓名： | 吴珊 李小芳 舒予 刘玲 文怡静 罗佳 |
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| 作者单位： | 成都中医药大学药学院中药材标准化教育部重点实验室中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137 |
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| 基金项目： | 四川省教育厅科研项目（编号：13ZB0315）;成都中医药大学科研基金（编号：ZRMS201240） |
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| 摘 要： | 目的：研究微波辅助提取山香圆叶总黄酮的最佳工艺条件。方法：以总黄酮得率为指标，采用单因素试验与正交试验相结合的方法，优化微波辅助提取山香圆叶中总黄酮的提取工艺条件。结果：微波辅助法提取山香圆总黄酮的最佳工艺条件为乙醇浓度70％，微波辐射时间为6min，微波功率为600W，料液比为1：40（g／mL）。在此条件下测得的提取率为1．606％。结论：微波辅助提取法可用于山香圆总黄酮的提取，其中乙醇浓度对总黄酮的提取有显著影响。
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| 关 键 词： | 山香圆叶 微波辅助提取 总黄酮 |
Optimization of Microwave-Ultrasound Assisted Extraction of Flavonoids from Leaves of Turpiniae Folium by Orthogonal Design |
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| Institution: | WU Shan, LI Xiao-fang, SHU Yu, et al ( Chengdu University of TCM Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicines System of Traditional Chinese Medicine Resources and Development Utilization of Ministry of State Key Laboratory Breeding Base, Chengdu, 611137, China) |
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| Abstract: | Objective： To study extraction technology of flavonoids from the leaves of Turpinia arguta Seem. by microwave method. Methods ： Taking extraction rate of flavonoids as index, singer-factor and orthogonal test were applied to optimize the extrac- tion technology of flavonoids from the leaves of Turpinia arguta Seem. by microwave method. Results ： The optimum extraction technolo- gy of flavonoids was as follows ： ethanol concenteation of 70 %, microwave treatment time of 6 min, microwave power of 600 w and solid-liquid of 1： 40. Under this condition, the extraction rate of flavonoids was 1. 606 %. Conclusion： The microwave method that was used to extract the flavonoids from Turpinia arguta Seem. Leaves is very effective, ethanol concenteation is the main influencing factor that affects extraction rate of flavonoids. |
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| Keywords: | Turpiniae Folium microwave method total flavonoids |
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