VEGF、核内EGFR及CD 105在早期非小细胞肺癌的表达及临床意义研究

目的：探讨 VEGF、核内 EGFR及CD105在早期非小细胞肺癌的表达情况及其与早期非小细胞肺癌临床病理特征的关系。方法选择30例手术切除的早期非小细胞肺癌组织及癌旁正常组织，应用免疫组化方法检测 VEGF、核内 EGFR及CD105的表达情况，分析3者之间的相互关系及临床意义。计量资料采用t检验，计数资料用卡方检验或四格表确切概率检验，两者相关性采用非参数Spearman等级相关性分析。结果 VEGF在30例早期非小细胞肺癌组织和癌旁正常组织中的阳性表达率分别为53．5％和26．7％，差异具有统计学意义（P ＜0．05）。核内 EGFR在癌组织和癌旁正常组织中的阳性表达率分别为16．7％和0％，差异无统计学意义（P ＞0．05），CD105标记的微血管密度值（MVD）在癌组织与癌旁正常组织中分别为（17．3±5．6）和（8．9±4．2），差异具有统计学意义（P ＜0．05）。VEGF与核内 EGFR的表达与肿瘤复发转移有关，差异具有统计学意义（P ＜0．05）， VEGF、核内 EGFR的表达及CD105标记的 MVD值与与患者年龄、性别、吸烟、肿块大小、病理类型、临床分期、分化程度、解剖学类型差异无统计学意义（P ＞0．05）。VEGF在癌组织中的表达与 MVD呈正相关性（r ＝0．516，P＝0．004），VEGF的阳性表达与核内 EGFR 的阳性表达、核内 EGFR 的阳性表达与 MVD 之间无相关性（P ＞0．05）。结论 VEGF、核内 EGFR及CD105在早期 NSCLC均有表达，VEGF、核内 EGFR的表达与早期非小细胞肺癌的复发转移有关。

Expression of VEGF,nuclear EGFR and CD105 in early stage non-small cell lung cancer

Objective To detect the expressions of vascular endothelial growth factor （VEGF）,nuclear epi-dermal growth factor receptor （EGFR）and CD105 in the early non-small cell lung cancer and analyze the relationship of these indices with the clinicopathologic features of early stage NSCLC.Methods The ex-pression status of VEGF,nuclear EGFR and CD105 were examined by immunohistochemistric method （IHC）in 30 cases of early-stage non-small cell lung cancer and adjacent normal tissue.Results The posi-tive rates of VEGF of 30 patients with early stage non-small cell lung cancer and adjacent normal tissue were 53.5% and 26.7% respectively,and the difference was statistically significant （P 〈0.05）.The posi-tive rates of nuclear EGFR of 30 patients with early stage non-small cell lung cancer and adjacent normal tissue were 16.7% and 0% respectively,and the difference was not statistically significant （P 〈0.05）. MVD marked by CD105 of 30 patients with early stage non-small cell lung cancer and adjacent normal tis-sue were （17.3±5.6）and （8.9±4.2）in the adjacent tissues and normal tissues,and the difference was sta-tistically significant （P 〈0.05）.The expression of VEGF and nuclear EGFR were significantly correlated with tumor recurrence and metastasis （r=0.516,P =0.004）;The expression of VEGF and nucleus EG-FR,as well as the expression of nucleus EGFR and MVD were not significantly correlated （P 〉0.05）. Conclusion The expressions of VEGF,nuclear EGFR and CD105 played an important role in the develop-ment of early-stage non-small cell lung cancer,and the expressions of VEGF and nuclear EGFR were rele-vant to the recurrence of early-stage non-small cell lung cancer.