养形与养神不同运动训练对SHR/SP大鼠血管性痴呆模型认知能力及血压的影响

目的 观察养形与养神不同运动训练对自发性高血压脑卒中倾向大鼠（SHR/SP）血管性痴呆模型认知能力及血压的影响.方法 选用雄性SHR/SP大鼠采用双侧颈总动脉永久性结扎（2-VO）法制作血管性痴呆（VD）模型,然后随机分为4组,分别为模型组,养形组,养神组和形神共养组,每组各10只,养形组采用跑台训练,养神组采用丰富环境饲养,形神共养组采用跑台训练结合丰富环境干预,模型组造模后正常环境饲养不做干预.8周后采用Morris水迷宫检测大鼠认知功能,同时尾套法检测血压干预前后的变化.结果 在定位航行训练中,形神共养组大鼠的潜伏期明显少于其他各组,养形组和养神组大鼠的潜伏期明显短于模型组,但养形组大鼠的潜伏期与养神组大鼠比无统计学差异（P＞0.05）;在空间探索实验中,形神共养组大鼠跨越原平台次数、目标象限探索时间百分比均明显多于其他各组,养形组和养神组大鼠跨越原平台次数、目标象限探索时间百分比均明显多于模型组,但养形组大鼠与养神组大鼠比无统计学差异（P＞0.05）;形神共养组大鼠的血压明显低于其他各组,养形组大鼠的血压明显低于模型组和养神组,养神组大鼠的血压较模型组有降低的趋势,但差异没有统计学意义（P＞0.05）.结论 形神共养理论指导下的训练可有效改善SHR／SP大鼠VD模型认知能力,同时有效降低高血压危险因素在VD发病中的作用.

Effect of Body Building and Spirit Fostering Exercise Training on Cognitive Function and Blood Pressure in SHR/SP Rat with Vascular Dementia

Objective To investigate the effect of body building and spirit fostering exercise training on the cognitive function and blood pressure in the Stroke Prone spontaneously hypertensive rat（SHR/ SP） with vascular dementia （VD）. Method The VD model was established by a permanent ligation of bilateral carotid artery （2-VO） in male SHR/SP rats. The VD model rats were randomly divided into model group（M group）, body building group（B group）, spirit fostering group（S group ） and body building ＆ spirit fostering group（BS group） （n=l 0） respectively. The B group was trained by using treadmill training, S group was trained by using enriched environment, BS group was trained by using treadmill training and enriched environment. After 8 weeks, Morris maze tests were conducted for valuating the learning- memory abilities, the blood pressure was measured using a tail cuff method. Result In place navigation training, the latency of BS group was less than other groups, the latency of B group and S group were less than that of rats in model group, there was not different between B group and S group （P〈0.05）; In spatial probe ability test, the frequency of crossing platform and the ratio of time spent in the target quadran of rats in BS group were significantly higher than other groups, the frequency of crossing platform and The ratio of time spent in the target quadran of rats in B group and S group were higher than M group （P〈0.05）, there was not different between B group and S group （P〈0.05）; the blood pressure in BS group was lower than other groups, the blood pressure in B group was lower than S groups and M group, there was not different between S group and M group （P〈0.05）. Conclusion Combination of the spirit and body exercise training is one of the important thoughts of the basic TCM theory, the training under guidance of the theory can improve the cognitive function and blood pressure in SHR/SP rat VD model.