脑牵拉引起脑神经元凋亡的实验研究

目的探讨脑牵拉压（BRP）的测量方法及脑牵拉压引起神经元细胞凋亡的规律和机制.方法利用电阻应变计制作脑牵拉力的测量装置,对其定标.选用新西兰大白兔30只,分为30、40、50 g组,牵拉完毕后,用流式细胞仪检测各组脑牵拉压区神经细胞凋亡的百分率和细胞线粒体的活性变化.结果30 g的BRP牵拉30 min引起较低的细胞凋亡率,几乎不影响细胞线粒体的膜电位;40 g的BRP牵拉30 min引起较高的细胞凋亡率.细胞线粒体的膜电位明显降低;50 g的BRP牵拉15 min引起更高的细胞凋亡率,细胞线粒体的膜电位显著降低.结论该装置可作为脑牵拉压的测量工具,脑牵拉可引起神经元细胞凋亡以及细胞线粒体膜电位降低.实验性脑牵拉时,最好将BRP控制在40 g以下.

Experimental study of neuron apoptosis induced by brain retraction

Objective To investigate the method of measurement of brain retraction pressure (BRP) and observe the risks of the neuron apoptosis and the decrease of mitochondrial membrance potential(MMP) induced by brain retraction. Methods Thirty rabbits were randomly divided into 30 g (the brain retracted by 30g ), 40 g and 50 g groups. BRP was determined by self-made BRP instrument. The percentage of neuron apoptosis and the decrease of MMP were measured by flow cytometry. Results The mild percentage of neuron apoptosis and slight decrease of MMP were observed in 30 g and 40 g groups after retraction lasting 30 min. The significant percentage of neuron apoptosis and significant decrease of MMP had been seen after retraction lasting 15 min in 50 g group. Conclusion This instrument can be used as a tool for measuring BRP. The brain retraction can induce neuron apoptosis and the decrease of MMP. The BRP during the experimental brain surgery should be no more than 40 g to avoid brain injuries induced by the retraction.