肝细胞生长因子及其受体c-Met在肾细胞癌组织中的表达

目的　探讨肝细胞生长因子 (HGF)及其受体c Met在肾细胞癌中表达的临床意义。方法　应用Northernblot方法检测 2 6例肾细胞癌和 10例非癌症肾组织HGFmRNA和c MetmRNA的表达情况。同时以GAPDH作为内参照。结果　 2 6例肾癌标本中 ,HGFmRNA及c MetmRNA表达均为阳性者 2 1例 ,占 80 .8%。临床病期分析结果显示 ,肾癌组织中TNM分期越高 ,HGFmRNA和c MetmRNA的表达越强烈 ,而且c MetmRNA表达量高于HGFmRNA。但对照组中的HGFmRNA和c MetmRNA均只有弱表达。结论　HGFmRNA及其受体c MetmRNA在肾细胞癌的生长、浸润和转移过程中可能起重要作用 ,对肾脏恶性肿瘤的诊断和预后的判定 ,可能具有潜在的临床价值

Expression of HGF and its receptor c-Met in renal cell carcinoma

Objective To study the clinical significance of expression of hepatic growth factor (HGF) and its receptor c Met in renal cell cancer (RCC). Methods Twenty six patients with RCC and 10 benign renal tumor patients were examined. The expression of HGF and c Met mRNA was detected using Northern blot. GAPDH was used as the internal control. Results Twenty one out of 26 patients with RCC had positive gene expression of HGF and c Met with the positive rate of 80.8%. According to the tumor TNM staging, the more advanced the cancer had a stronger expression of HGF and c Met, the expression of c Met was higher than that of HGF in the RCC. Whereas in the control group with benign tumor, the expression of c Met was rather mild. Conclusion The result suggests that HGF and its receptor c Met may play an important role in the development and progression of renal cancer, which may also imply a potential clinical significance in diagnosing and assessing prognosis of RCC.