小活络丸中胆南星的薄层色谱鉴别方法

目的：建立小活络丸中胆酸、猪去氧胆酸和鹅去氧胆酸的薄层色谱鉴别方法，用以帮助提高胆南星的鉴别手段。方法：采用薄层色谱方法，在异辛烷-乙醚．冰醋酸-正丁醇－水（10：5：5：3：1）上层溶液的展开条件下对所抽取45批样品进行实验。结果：经过对13个被抽样单位共45批样品的薄层色谱结果进行汇总统计分析，有73．33％的样品捡出胆酸，86．67％的样品检出猪去氧胆酸，93．33％的样品检出鹅去氧胆酸。结论：建立的胆酸、猪去氧胆酸和鹅去氧胆酸的薄层色谱鉴别方法，可作为小活络丸中胆南星鉴别的辅助手段。

Identification of Arisaema Cum Bile in Small Meridian-Activating Pill by Thin Layer Chromatography

OBJECTIVE：To identify cholic acid, hyodeoxycholic acid and chenodeoxycholic acid in small meridian- activating pill by thin layer chromatography （TLC） in an effort to improve the means for identification of arisaema cum bile. METHODS ：45 batches of samples were identified by TLC with the upper solution of isooctane-diethyl ether-acetic acid-butanol-water （ 10：5 ： 5 ： 3 ： 1 ） as developer. RESULTS： In the 45 batches of samples from 13 manufactories identified, eholie acid was detected in 73.33% samples, hyodeoxycholic acid in 86.67% samples and ehenodeoxycholic acid in 93.33%. CONCLUSION：The established TLC for identification of eholie acid, hyodeoxycholie acid and ehenodeoxycholic acid could be used as supplementary means for identification of arisaema cum bile in small meridian-activating pill.