气相色谱法测定甘草药材中9种有机氯农药的残留量

目的：建立同时测定甘草药材中9种有机氯农药残留量的气相色谱法。方法：采用气相色谱法,毛细管色谱柱DB-1701（30 m×0.25 mm,0.25μm）,进样口：220℃,柱温：初始140℃保持1 min,以10℃/min的速度升至210℃保持1 min,再以20℃/min的速度升至260℃保持20 min;检测器：300℃;载气为高纯氮（纯度〉99.999%）;载气流速：1.0 ml/min,恒流模式,不分流,进样量1μl。结果：9种有机氯农药在1～250 ng/ml浓度范围内呈良好线性关系;加样回收率平均为102.40%,RSD为2.12%（n=9）。结论：本方法简便、快速、准确,可用于测定甘草药材中9种有机氯农药的残留量。

Determination of 90rganochlorine Pesticides in Licorice Root Medicinal Material by Gas Chromatography

OBJECTIVE：To establish gas chromatography（GC） for simultaneous determination of the contents of 9 organochlorine pesticides in licorice root medicinal material. METHODS：The determination of contents was performed by GC,briefly,capillary column DB-1701 （30 m ×0.25 mm,0.25μm）was used,with injection port temperature kept at 220 ℃;column temperature kept at 140 ℃ for 1 min then raised to 210 ℃ at an increasing rate of 10 ℃/min,with the temperature retained for 1 rain then raised to 260 ℃at an increasing rate of 20 ℃/min, with this temperature retained for 20 rain;the detector temperature was kept at 300 ℃ ;the carrier gas was high purity nitrogen （ 〉 99. 999% ） at a flow rate of 1.0 ml/min in constant current mode without split flow with injection volume of 1 μl. RESULTS：The linear ranges of the 9 organochlorine pesticides were 1-250 ng/ml. The average recovery rate was 102.40% with RSD of 2. 12% （n = 9）. CONCLUSION： The GC method is simple, rapid and accurate, and it is suitable for the content determination of 9 organochlorine pesticides in licorice root medicinal material.